SIGLEC7 抗体 (N-Term)
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Quick Overview for SIGLEC7 抗体 (N-Term) (ABIN357147)
抗原
See all SIGLEC7 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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特异性
- This antibody detects SIGLEC7 at N-term.
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纯化方法
- Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
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免疫原
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human SIGLEC7.
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亚型
- Ig Fraction
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anti-Sialic Acid Binding Ig-Like Lectin 7 (SIGLEC7) (AA 377-467) antibody
SIGLEC7 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Sialic Acid Binding Ig-Like Lectin 7 (SIGLEC7) (AA 38-121) antibodySIGLEC7 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Sialic Acid Binding Ig-Like Lectin 7 (SIGLEC7) antibody (PE)SIGLEC7 适用: 人 FACS 宿主: 小鼠 Monoclonal 6-434 PE
anti-Sialic Acid Binding Ig-Like Lectin 7 (SIGLEC7) antibodySIGLEC7 适用: 人 WB, ICC, IHC (p), IF 宿主: 兔 Polyclonal unconjugated
anti-Sialic Acid Binding Ig-Like Lectin 7 (SIGLEC7) (AA 18-353) antibodySIGLEC7 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C5 unconjugated
anti-Sialic Acid Binding Ig-Like Lectin 7 (SIGLEC7) (AA 1-467) antibodySIGLEC7 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3H1 unconjugated
anti-Sialic Acid Binding Ig-Like Lectin 7 (SIGLEC7) antibodySIGLEC7 适用: 人 FACS 宿主: 小鼠 Monoclonal 6-434 unconjugated
anti-Sialic Acid Binding Ig-Like Lectin 7 (SIGLEC7) (AA 18-353) antibodySIGLEC7 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Sialic Acid Binding Ig-Like Lectin 7 (SIGLEC7) (AA 1-30), (N-Term) antibodySIGLEC7 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB2711 unconjugated
anti-Sialic Acid Binding Ig-Like Lectin 7 (SIGLEC7) (AA 407-438) antibodySIGLEC7 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA 1: 1,000. Western blot 1: 100 - 1: 500. Immunohistochemistry 1: 50 - 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.25 mg/mL
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缓冲液
- PBS with 0.09 % (W/V) sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
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- SIGLEC7 (Sialic Acid Binding Ig-Like Lectin 7 (SIGLEC7))
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别名
- CD328 / SIGLEC7
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背景
- SIGLECs are members of the immunoglobulin superfamily that are expressed on the cell surface. Most SIGLECs have one or more cytoplasmic immune receptor tyrosine-based inhibitory motifs (ITIM). SIGLECs are typically expressed on cells of the innate immune system, with the exception of the B-cell expressed SIGLEC6. Sequence analysis predicted that the 697-amino acid SIGLEC10 protein contains a signal peptide, an N-terminal V-set Ig-like domain and four C2-set Ig-like domains, five potential N-linked glycosylation sites, a transmembrane region, and a 126-residue cytoplasmic tail with 3 putative ITIMs. Northern blot analysis detected a major 3.0-kb SIGLEC10 transcript, with highest levels in spleen, lymph node, blood leukocytes, and appendix. Little or no expression was observed in pancreas, thyroid, and testis. Flow cytometric analysis demonstrated eosinophil-specific expression of SIGLEC10, but at a lower level than that of SIGLEC8. Expression was also detected on monocytes and a CD16-positive/CD56-negative natural killer-like lymphocyte population. After sialidase treatment, which is necessary for unmasking the sialic acid-binding site on SIGLECs interacting with cell surface sialic acids, cells expressing SIGLEC10 bound to red blood cells. Immunoprecipitation analysis indicated expression of a 100- to 120-kD monomeric protein, higher than the predicted molecular mass, suggesting that SIGLEC10 is glycosylated.Synonyms: AIRM1, Adhesion inhibitory receptor molecule 1, CDw328, D-siglec, QA79 membrane protein, Sialic acid-binding Ig-like lectin 7, Siglec-7, p75
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分子量
- 51142 Da
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基因ID
- 27036, 5874
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UniProt
- Q9Y286
抗原
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