GFP 抗体
(8 references)Quick Overview for GFP 抗体 (ABIN356346)
抗原
See all GFP 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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产品特性
- Synonyms: Green fluorescent protein, GFP-Tag
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纯化方法
- Protein G Chromatography eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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免疫原
- Purified His-tagged GFP protein was used to produced this monoclonal antibody.
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亚型
- IgG1
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anti-Green Fluorescent Protein (GFP) antibody
GFP 适用: Aequorea victoria WB, ELISA, FM 宿主: 山羊 Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea aequorea WB, IF, IHC (fro), IHC (p) 宿主: 山羊 Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, ELISA, IP, IF, FACS 宿主: 小鼠 Monoclonal 6C11C11 unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, ELISA, IP, IC, ChIP-seq 宿主: 大鼠 Monoclonal 1A5 unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, IF, IHC (fro), IHC (p), IEM 宿主: 山羊 Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, IHC, IP, ICC, FACS 宿主: 小鼠 Monoclonal 4B10B2 unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, ELISA, IHC, IP, FM, FACS, DB 宿主: 小鼠 Monoclonal 9F9-F9 unconjugated
anti-Green Fluorescent Protein (GFP) antibody (DyLight 550)GFP 适用: Aequorea victoria WB, IF, IHC (fro) 宿主: 山羊 Polyclonal DyLight 550
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB, ELISA, IHC, IP, FM, FACS 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: 1/3,000. Western blotting: 1/2,000. Immunohistochemistry: 1/50-1/100. Immunofluorescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.25 mg/mL
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缓冲液
- PBS containing 0.09 % (w/v) Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
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Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. -
有效期
- 12 months
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: "Perturbations in dopamine synthesis lead to discrete physiological effects and impact oxidative stress response in Drosophila." in: Journal of insect physiology, Vol. 73, pp. 11-9, (2015) (PubMed).
: "GMP-compliant isolation and large-scale expansion of bone marrow-derived MSC." in: PLoS ONE, Vol. 7, Issue 8, pp. e43255, (2012) (PubMed).
: "Mechanism of a genetically encoded dark-to-bright reporter for caspase activity." in: The Journal of biological chemistry, Vol. 286, Issue 28, pp. 24977-86, (2011) (PubMed).
: "Proteolytic cleavage of human acid-sensing ion channel 1 by the serine protease matriptase." in: The Journal of biological chemistry, Vol. 285, Issue 35, pp. 27130-43, (2010) (PubMed).
: "The unique hypusine modification of eIF5A promotes islet beta cell inflammation and dysfunction in mice." in: The Journal of clinical investigation, Vol. 120, Issue 6, pp. 2156-70, (2010) (PubMed).
: "Kv4 accessory protein DPPX (DPP6) is a critical regulator of membrane excitability in hippocampal CA1 pyramidal neurons." in: Journal of neurophysiology, Vol. 100, Issue 4, pp. 1835-47, (2008) (PubMed).
: "Localization of the phosphoethanolamine methyltransferase of the human malaria parasite Plasmodium falciparum to the Golgi apparatus." in: The Journal of biological chemistry, Vol. 281, Issue 30, pp. 21305-11, (2006) (PubMed).
: "Identification of a Cys motif in the common beta chain of the interleukin 3, granulocyte-macrophage colony-stimulating factor, and interleukin 5 receptors essential for disulfide-linked receptor ..." in: The Journal of biological chemistry, Vol. 273, Issue 2, pp. 1192-9, (1998) (PubMed).
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: "Perturbations in dopamine synthesis lead to discrete physiological effects and impact oxidative stress response in Drosophila." in: Journal of insect physiology, Vol. 73, pp. 11-9, (2015) (PubMed).
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- GFP (Green Fluorescent Protein (GFP))
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别名
- GFP
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背景
- Green fluorescent protein (GFP), originally isolated from the jellyfish Aequorea victoria, is one of the best visual reporters for monitoring gene expression in vivo and in situ. GFP is a also convenient marker for use in flow cytometry because it eliminates the need to incubate with asecondary reagent (such as dyes or antibodies) for detection. However, anti-GFP antibody is also widely used for co-immunoprecipitation, co-localization or western blotting for the confirmation of specificity when a GFP fusion protein is expressed in cells. This monoclonal antibody provides a simple solution to detect the expression of a GFP-tagged protein in cells. Because of its ability to spontaneously generate its own fluorophore, the green fluorescent protein (GFP) from the jellyfish Aequorea victoria is used extensively as a fluorescent marker in molecular and cell biology. The yellow fluorescent proteins (YFPs) have the longest wavelength emissions of all GFP variants examined to date. This shift in the spectrum is the result of a T203Y substitution (single-letter amino acid code), a mutation rationally designed on the basis of the X-ray structure of GFP S65T. Anti-GFP monoclonal antibody can detect both GFP and YFP but not BFP (Blue fluorescent protein) by western blotting.Synonyms: GFP-Tag, Green fluorescent protein
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UniProt
- P42212
抗原
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