ERK1/2 抗体
Quick Overview for ERK1/2 抗体 (ABIN351562)
抗原
See all ERK1/2 (MAPK1/3) 抗体适用
宿主
克隆类型
标记
应用范围
-
-
特异性
- Detects endogenous levels of total p44/42 MAP kinase (Erk1/Erk2) protein. In some cell types, recognizes p42 MAPK more readily than p44 MAPK. Does not recognize JNK/SAPK nor p38 MAP kinase. Species cross-reactivity: Human, mouse, rat, hamster, zebrafish.
-
纯化方法
- Immunoaffinity purified
-
免疫原
-
Synthetic peptide (KLH coupled) derived from the sequence of rat p42 MAP kinase.
Type of Immunogen: Synthetic peptide -
亚型
- IgG
-
-
-
-
应用备注
-
Approved: ELISA, Flo (1:25), ICC, IF, IHC, IHC-P, IP (1:50), WB (1:1000)
Usage: Suitable for use in Immunofluorescence, Immunocytochemistry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Flow Cytometry. Western Blot: 1:1000, Incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4?C with gentle shaking, overnight. Immunohistochemistry (Paraffin sections): 1:100. Immunoprecipitation: 1:50. Immunocytochemistry (IF): 1:25. Flow Cytometry: 1:25. -
说明
-
Target Species of Antibody: Rat
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- Lot specific
-
缓冲液
- 10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.
-
储存液
- Without preservative
-
注意事项
- Avoid repeat freeze-thaw cycles.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
-
-
- ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))
-
别名
- ERK1 + ERK2
-
背景
-
Name/Gene ID: ERK1/ERK2
Synonyms: ERK1/ERK2
This antibody detects: ERK1 + ERK2
抗原
-