ERK1/2 抗体 (pThr202, pTyr204)
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- 抗原 See all ERK1/2 (MAPK1/3) 抗体
- ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))
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抗原表位
- pThr202, pTyr204
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This ERK1/2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC)
- 原理
- Rabbit antibody to phospho T202 & Y204 Erk1 Erk2
- 特异性
- Specific for Erk1 and Erk2.
- 交叉反应
- 人, 小鼠, 大鼠
- 交叉反应 (详细)
- Other species not yet tested.
- 纯化方法
- IgG
- 免疫原
- A synthetic peptide from the phospho T202 & Y204 of human Erk1 Erk2 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in many species including rat mouse zebra fish xenopus and chicken.
- 亚型
- IgG
- Top Product
- Discover our top product MAPK1/3 Primary Antibody
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- 应用备注
- IHC WB. A concentration of 10-50 μg,ml is recommended. The optimal concentration should be determined by the end user. This antibody may also recognise the unphosphorylated form. Not yet tested in other applications.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
- 注意事项
- Avoid freeze and thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
- 有效期
- 12 months
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- 抗原
- ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))
- 别名
- Erk1 Erk2 (MAPK1/3 产品)
- 背景
- FUNCTION: Involved in both the initiation and regulation of meiosis mitosis and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1, required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1. Phosphorylates heat shock factor protein 4 (HSF4). CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein. COFACTOR: Magnesium. ENZYME REGULATION: Activated by tyrosine phosphorylation in response to insulin and NGF. SUBUNIT: Interacts with MORG1. Binds to HIV-1 Nef. This interaction inhibits its kinase activity. Interacts with HSF4 and NISCH.
- UniProt
- P27361
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