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GFP 抗体

GFP 适用: Aequorea victoria WB, IHC 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN350304
发货至: 中国
  • 抗原 See all GFP 抗体
    GFP (Green Fluorescent Protein (GFP))
    适用
    • 161
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    • 5
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    Aequorea victoria
    宿主
    • 67
    • 61
    • 35
    • 14
    • 5
    • 5
    • 2
    • 1
    • 1
    克隆类型
    • 111
    • 76
    多克隆
    标记
    • 85
    • 11
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    • 5
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    This GFP antibody is un-conjugated
    应用范围
    • 148
    • 81
    • 61
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    • 32
    • 30
    • 30
    • 20
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    • 13
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    Western Blotting (WB), Immunohistochemistry (IHC)
    原理
    Rabbit antibody to EGFP/GFP/Venus
    特异性
    Specific for EGFP/GFP/Venus
    交叉反应 (详细)
    Any organism expressing EGFP/GFP/Venus.
    纯化方法
    IgG
    免疫原
    Recombinant EGFP/GFP/Venus protein was used as the antigen.
    亚型
    IgG
    Top Product
    Discover our top product GFP Primary Antibody
  • 应用备注
    IHC WB. A working concentration of 5-50 μg,ml is recommended. The optimal concentration should be determined by the end user.
    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
    注意事项
    Avoid freeze and thaw cycles.
    储存条件
    4 °C,-20 °C
    储存方法
    Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term.
    有效期
    12 months
  • 抗原
    GFP (Green Fluorescent Protein (GFP))
    Abstract
    GFP 产品
    背景
    GFP is a naturally fluorescent protein discovered by chance in the 1960s by Shimomura et al. GFP makes green light out of  aequorin's blue light. The chromophore is a modified Ser-Tyr-Gly sequence. GFP can emit light once this short tripeptide has adopted a cyclic conformation with the help of a oxygen. BIOPHYSICOCHEMICAL PROPERTIES: Excitation max (nm): 488; Emission max (nm): 509; Extinction coefficient (Cm-1M-1): 61000. Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer reporter of gene expression or as a measure of protein-protein interactions.
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