GFP 抗体
Quick Overview for GFP 抗体 (ABIN350302)
抗原
See all GFP 抗体适用
宿主
克隆类型
标记
应用范围
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原理
 - Sheep antibody to EGFP/GFP/Venus
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特异性
 - Specific for EGFP/GFP/Venus
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交叉反应 (详细)
 - Any organism expressing EGFP/GFP/Venus.
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纯化方法
 - IgG
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免疫原
 - Recombinant EGFP/GFP/Venus protein was used as the antigen.
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亚型
 - IgG
 
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应用备注
 - IHC WB. Use at a concentration of 10-50 μg,ml. The optimal working concentration should be determined by the end user.
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限制
 - 仅限研究用
 
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状态
 - Lyophilized
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溶解方式
 - Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
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注意事项
 - Avoid freeze and thaw cycles.
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储存条件
 - 4 °C,-20 °C
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储存方法
 - Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
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有效期
 - 12 months
 
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                                            : "Amyloid β-induced elevation of O-GlcNAcylated c-Fos promotes neuronal cell death."    in: Aging cell, Vol. 18, Issue 1, pp. e12872, (2020)         (PubMed).        
 
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                                            : "Amyloid β-induced elevation of O-GlcNAcylated c-Fos promotes neuronal cell death."    in: Aging cell, Vol. 18, Issue 1, pp. e12872, (2020)         (PubMed).        
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- GFP (Green Fluorescent Protein (GFP))
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背景
 - GFP is anaturally fluorescent protein discovered by chance in the 1960s by Shimomura et al. BIOPHYSICOCHEMICAL PROPERTIES: Excitation max (nm): 488, Emission max (nm): 509, Extinction coefficient (Cm-1M-1): 61000. Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins.
 
抗原
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