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GFP 抗体

Cited in 1 publication. This anti-GFP antibody is a 绵羊 多克隆 antibody detecting GFP in WB 和 IHC. Suitable for Aequorea victoria.
产品编号 ABIN350302
发货至: 中国
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中国
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for GFP 抗体 (ABIN350302)

抗原

See all GFP 抗体
GFP (Green Fluorescent Protein (GFP))

适用

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Aequorea victoria

宿主

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绵羊

克隆类型

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多克隆

标记

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This GFP antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC)
  • 原理

    Sheep antibody to EGFP/GFP/Venus

    特异性

    Specific for EGFP/GFP/Venus

    交叉反应 (详细)

    Any organism expressing EGFP/GFP/Venus.

    纯化方法

    IgG

    免疫原

    Recombinant EGFP/GFP/Venus protein was used as the antigen.

    亚型

    IgG
  • 应用备注

    IHC WB. Use at a concentration of 10-50 μg,ml. The optimal working concentration should be determined by the end user.

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.

    注意事项

    Avoid freeze and thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

    有效期

    12 months
  • Choi, Kim, Song, Cha, Cho, Son, Kim, Mook-Jung: "Amyloid β-induced elevation of O-GlcNAcylated c-Fos promotes neuronal cell death." in: Aging cell, Vol. 18, Issue 1, pp. e12872, (2020) (PubMed).

  • 抗原

    GFP (Green Fluorescent Protein (GFP))

    背景

    GFP is anaturally fluorescent protein discovered by chance in the 1960s by Shimomura et al. BIOPHYSICOCHEMICAL PROPERTIES: Excitation max (nm): 488, Emission max (nm): 509, Extinction coefficient (Cm-1M-1): 61000. Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins.
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