GFP 抗体
Quick Overview for GFP 抗体 (ABIN350302)
抗原
See all GFP 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Sheep antibody to EGFP/GFP/Venus
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特异性
- Specific for EGFP/GFP/Venus
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交叉反应 (详细)
- Any organism expressing EGFP/GFP/Venus.
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纯化方法
- IgG
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免疫原
- Recombinant EGFP/GFP/Venus protein was used as the antigen.
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亚型
- IgG
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应用备注
- IHC WB. Use at a concentration of 10-50 μg,ml. The optimal working concentration should be determined by the end user.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
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注意事项
- Avoid freeze and thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
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有效期
- 12 months
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: "Amyloid β-induced elevation of O-GlcNAcylated c-Fos promotes neuronal cell death." in: Aging cell, Vol. 18, Issue 1, pp. e12872, (2020) (PubMed).
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: "Amyloid β-induced elevation of O-GlcNAcylated c-Fos promotes neuronal cell death." in: Aging cell, Vol. 18, Issue 1, pp. e12872, (2020) (PubMed).
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- GFP (Green Fluorescent Protein (GFP))
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背景
- GFP is anaturally fluorescent protein discovered by chance in the 1960s by Shimomura et al. BIOPHYSICOCHEMICAL PROPERTIES: Excitation max (nm): 488, Emission max (nm): 509, Extinction coefficient (Cm-1M-1): 61000. Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins.
抗原
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