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ATG3 抗体

This anti-ATG3 antibody is a 绵羊 多克隆 antibody detecting ATG3 in WB 和 IHC. Suitable for 人.
产品编号 ABIN349972
发货至: 中国

Quick Overview for ATG3 抗体 (ABIN349972)

抗原

See all ATG3 抗体
ATG3 (ATG3 Autophagy Related 3 (ATG3))

适用

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宿主

  • 90
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绵羊

克隆类型

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多克隆

标记

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This ATG3 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC)
  • 原理

    Sheep antibody to ATG3

    特异性

    Specific for ATG3.

    交叉反应

    人, 小鼠, 大鼠

    交叉反应 (详细)

    Other species not yet tested.

    纯化方法

    IgG

    免疫原

    A synthetic peptide from human ATG3 conjugated to an immunogenic carrier protein has been used as the antigen. The antigen is homologous in rat and mouse.

    亚型

    IgG
  • 应用备注

    IHC WB. Use at a concentration of 10-50,micro,g,ml. The optimal working concentration should be determined by the end user. Not yet tested in other applications.

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.

    注意事项

    Avoid freeze and thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

    有效期

    12 months
  • 抗原

    ATG3 (ATG3 Autophagy Related 3 (ATG3))

    别名

    ATG3

    背景

    Autophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole. Human ATG3 displays the same enzymatic characteristics in vitro as yeast Apg3 a protein-conjugating enzyme essential for autophagy. FUNCTION: GABARAPL1 (GABARAPL2 or GABARAP or MAP1LC3)-modifier protein conjugating enzyme involved in its E2-like covalent binding to PE. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3 (E2-like enzyme). Preferred substrate is MAP1LC3A. Formation of the GABARAPL1-PE conjugate is essential for autophagy. SUBCELLULAR LOCATION: Cytoplasm TISSUE SPECIFICITY: Widely expressed with a highest expression in heart skeletal muscle kidney liver and placenta.

    UniProt

    Q9NT62

    途径

    Autophagy
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