ATG3 抗体
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北京 101111
Quick Overview for ATG3 抗体 (ABIN7698506)
抗原
See all ATG3 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Sheep antibody to ATG3
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特异性
- Specific for ATG3.
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交叉反应
- 人, 小鼠, 大鼠
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交叉反应 (详细)
- Other species not yet tested.
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纯化方法
- IgG
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免疫原
- A synthetic peptide from human ATG3 conjugated to an immunogenic carrier protein has been used as the antigen. The antigen is homologous in rat and mouse.
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亚型
- IgG
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应用备注
- IHC WB. Use at a concentration of 10-50,micro,g,ml. The optimal working concentration should be determined by the end user. Not yet tested in other applications.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute in 500 µl of sterile water. Centrifuge to remove any insoluble material.
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注意事项
- Avoid freeze and thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
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有效期
- 12 months
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- ATG3 (ATG3 Autophagy Related 3 (ATG3))
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别名
- ATG3
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背景
- Autophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole. Human ATG3 displays the same enzymatic characteristics in vitro as yeast Apg3 a protein-conjugating enzyme essential for autophagy. FUNCTION: GABARAPL1 (GABARAPL2 or GABARAP or MAP1LC3)-modifier protein conjugating enzyme involved in its E2-like covalent binding to PE. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3 (E2-like enzyme). Preferred substrate is MAP1LC3A. Formation of the GABARAPL1-PE conjugate is essential for autophagy. SUBCELLULAR LOCATION: Cytoplasm TISSUE SPECIFICITY: Widely expressed with a highest expression in heart skeletal muscle kidney liver and placenta.
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UniProt
- Q9NT62
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途径
- Autophagy
抗原
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