STAT5A 抗体 (pTyr694)
Quick Overview for STAT5A 抗体 (pTyr694) (ABIN349611)
抗原
See all STAT5A 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- pTyr694
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Supplier Product No.
- 200-301-a45
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Supplier
- Rockland
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原理
- Stat5 phospho Y694 Antibody
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交叉反应 （详细）
- This antibody is specific for mouse Stat5a protein phosphorylated at Y694.
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产品特性
- Synonyms: mouse anti-STAT5 pY694 Antibody, MGF antibody, Signal Transducer and Activator of Transcription 5A antibody, STAT 5 antibody
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纯化方法
- Phospho STAT5 pY694 was purified from concentrated tissue culture supernate by Protein A chromatography.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Anti-STAT5 phospho Y694 monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y694 of mouse STAT5a protein.
Immunogen Type: Conjugated Peptide
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亚型
- IgG1 kappa
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anti-Signal Transducer and Activator of Transcription 5A (STAT5A) antibody
STAT5A 适用: 人, 小鼠, 大鼠 WB, IHC (p), IF (cc) 宿主: 兔 Monoclonal 6E5 unconjugated
anti-Signal Transducer and Activator of Transcription 5A (STAT5A) (AA 583-794) antibodySTAT5A 适用: 人 WB, IHC, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 6D4 unconjugated
anti-Signal Transducer and Activator of Transcription 5A (STAT5A) (AA 1-794) antibodySTAT5A 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Signal Transducer and Activator of Transcription 5A (STAT5A) (AA 1-104) antibodySTAT5A 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 1B12 unconjugated
anti-Signal Transducer and Activator of Transcription 5A (STAT5A) (AA 635-794) antibodySTAT5A 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Signal Transducer and Activator of Transcription 5A (STAT5A) (C-Term) antibodySTAT5A 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Signal Transducer and Activator of Transcription 5A (STAT5A) (pTyr694) antibodySTAT5A 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Signal Transducer and Activator of Transcription 5A (STAT5A) (pSer780) antibodySTAT5A 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Signal Transducer and Activator of Transcription 5A (STAT5A) (AA 61-160) antibodySTAT5A 适用: 人, 小鼠, 大鼠, Cow WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Signal Transducer and Activator of Transcription 5A (STAT5A) (AA 763-797), (C-Term) antibodySTAT5A 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB52392 unconjugated
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应用备注
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Immunohistochemistry Dilution: 20-40 μg/mL
Application Note: Phospho STAT5 pY694 monoclonal antibody has been tested by ELISA, western blot, immunohistochemistry, and immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 91 kDa in size corresponding to phosphorylated Stat5a protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with mouse Stat5a pY694 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:20,000
IF Microscopy Dilution: 1:50-1:1000
Other: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store STAT5 phospho Y694 Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Phospho STAT5 antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))
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别名
- Stat5a
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背景
- Background: Signal transducer and activator of transcription 5 (Stat5) belongs to a family of cytoplasmic transcription factors that can be activated (phosphorylated) by a cell surface receptor. Phosphorylation at Tyr694 is obligatory for Stat5 activation. Stat5 has two isoforms, Stat5α and Stat5ß. Aberrant Stat5 activation has been implicated in the pathogenesis of chronic myelogenous leukemia, prostate and breast cancer and tumor metastasis. Stat5 is localized in the cytoplasm and upon phosphorylation at Y694 is translocated to the nucleus. Ideal for Cancer, Chromatin & Nuclear Signaling and Signal Transduction research.
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基因ID
- 20850, 6755672
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UniProt
- P42230
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途径
- JAK/STAT Signaling, RTK signaling, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation
抗原
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