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His Tag 抗体 (HRP)

Name: His Tag 抗体 (HRP)
SKU: ABIN349595
Availability: OutOfStock

Rockland 600-403-382 WB, ELISA, IHC 宿主: 兔 Polyclonal HRP

Anti-6X His epitope tag polyclonal antibody detects His-tagged recombinant proteins by western blot. (His Tag 抗体 (HRP))

1 image

(2 references)

产品编号 ABIN349595

Supplier Product No.: 600-403-382

发货至: 中国

Datasheet as PDF

抗原 See all His Tag 抗体

His Tag

适用

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宿主
95

59

11

9

9

8

2

1
兔
Compatible Secondaries
克隆类型
107

74

10
多克隆
标记
91

19

13

13

4

4

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

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1
This His Tag antibody is conjugated to HRP
应用范围
152

112

33

32

29

24

15

15

14

13

13

12

7

5

5

4

4

4

3

3

2

2

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Supplier Product No.

600-403-382

Supplier

Rockland

原理

6X His Epitope Tag Antibody Peroxidase Conjugated

序列

HHHHHH

交叉反应（详细）

This affinity purified antibody is directed against the 6X His motif and is useful in determining its presence in various assays. This polyclonal anti-6X His-tag antibody detects over-expressed proteins containing the 6X His epitope tag.

产品特性

Synonyms: rabbit anti-6X HIS EPITOPE TAG Antibody HRP conjugation, peroxidase conjugated rabbit anti-6X HIS EPITOPE TAG Antibody, anti-HIS, HIS Antibody, 6X His Tag Antibody, HHHHHH epitope tag antibody

纯化方法

Affinity purified antibody

免疫原

Immunogen: This antibody was purified from whole rabbit serum prepared by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
Immunogen Type: Conjugated Peptide

亚型

IgG

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WB, ELISA, FACS, DB, FM, FLISA 宿主: 小鼠 Monoclonal 33D10-D2-G8 Cy3

应用备注

Immunohistochemistry Dilution: 1:500 - 1:2,000
Application Note: Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, anti-6X His/6X His can be used to identify fusion proteins containing the 6X His epitope. The antibody recognizes the His tag fused either to the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and His-containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry.
Western Blot Dilution: 1:2,000 - 1:5,000
ELISA Dilution: 1:5,000
Other: User Optimized

限制

仅限研究用
状态

Lyophilized

溶解方式

Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)

浓度

1.0 mg/mL

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

储存液

Gentamicin sulfate

注意事项

This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

有效期

12 months
Pérez, Liu, Rodionov, Li, Bryant: "Complementation of Cobalamin Auxotrophy in Synechococcus sp. Strain PCC 7002 and Validation of a Putative Cobalamin Riboswitch In Vivo." in: Journal of bacteriology, Vol. 198, Issue 19, pp. 2743-52, (2017) (PubMed).

Yi, Ba, Zhang, Ho, Chen: "Single amino acid substitutions in the severe acute respiratory syndrome coronavirus spike glycoprotein determine viral entry and immunogenicity of a major neutralizing domain." in: Journal of virology, Vol. 79, Issue 18, pp. 11638-46, (2005) (PubMed).
抗原

His Tag

别名

6X His (His Tag 产品)

物质类

Tag

背景

Background: Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often, sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows Anti epitope tag antibodies to serve as universal detection reagents for any tag-containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.