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Keratin 5/8 抗体 (AA 353-367) (Biotin)

This anti-Keratin 5/8 antibody is a 小鼠 单克隆 antibody detecting Keratin 5/8 in FACS, IHC, IHC (p), IHC (fro) 和 ELISA. Suitable for 人.
产品编号 ABIN344035
发货至: 中国
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Quick Overview for Keratin 5/8 抗体 (AA 353-367) (Biotin) (ABIN344035)

抗原

Keratin 5/8 (KRT5/8)

适用

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宿主

  • 23
小鼠

克隆类型

  • 23
单克隆

标记

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This Keratin 5/8 antibody is conjugated to Biotin

应用范围

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Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA
  • 抗原表位

    AA 353-367

    特异性

    Reactivities on Cultured MCF-7, RT 112, HT-29, Detroit 562, RPMI 2650. Cell lines (tested so far) SSC-12, bovine BMGE+H, BMGE-H, MDBK. Represents an excellent marker for distinguishing carcinomas from all non-epithelial tumors. Specifically reacts with cytokeratins 5 and 8 present in nearly all epithelia. Polypeptide reacting: Mr 52500, Mr 58000 cytokeratins (type II cytokeratins nos. 5+8) of human epithelial cells. Epitope has been mapped to aa353-367 on alpha helical rod domain. Species cross-reactivity: Human, bovine, murine, amphibia, porcine, hydra.

    纯化方法

    Protein A purified

    免疫原

    Human cytokeratin 8, purified from SDS PAGE.

    Type of Immunogen: Purified protein

    亚型

    IgG1
  • 应用备注

    Approved: ELISA, Flo, IHC, IHC-Fr, IHC-P

    Usage: Suitable for use in ELISA, FACS and Immunohistochemistry (frozen and paraffin-embedded tissue and cytological material, Pretreatment With paraffin embedded sections, protease pretreatment is required prior to antibody application). Immunohistochemistry: 1:10-1:20, Incubation Time 1hr at RT, extended with paraffin sections (overnight at 2-8?C). The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 浓度

    Lot specific
  • 抗原

    Keratin 5/8 (KRT5/8)

    别名

    Cytokeratin 5+8

    背景

    This antibody detects: Cytokeratin 5+8
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