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CD40 抗体

This anti-CD40 antibody is a 小鼠 单克隆 antibody detecting CD40 in FACS, IHC, IP, IHC (p) 和 IHC (fro). Suitable for 人 和 犬.
产品编号 ABIN337317
发货至: 中国

Quick Overview for CD40 抗体 (ABIN337317)

抗原

See all CD40 抗体
CD40

适用

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人, 犬

宿主

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小鼠

克隆类型

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单克隆

标记

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This CD40 antibody is un-conjugated

应用范围

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Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

克隆位点

LOB7-6
  • 品牌

    IHC-plus™

    特异性

    Recognizes the human CD40 cell surface antigen, a 48kD glycoprotein expressed by B lymphocytes and weakly by some monocytes. CD40 is involved in the process of B cell selection in germinal centers and is vital in T cell-B cell interactions.

    纯化方法

    Protein A purified

    免疫原



    Type of Immunogen: Native protein

    亚型

    IgG2a
  • 应用备注

    Approved: Flo (20 μg/mL), IHC, IHC-Fr (1 - 10 μg/mL), IHC-P (10 μg/mL), IP

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry: This product requires protein digestion pre-treatment of paraffin embedded sections prior to staining e. g. pronase or trypsin. Histology Positive Control Tissue: Human Tonsil.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    Tris buffered saline, 0.09 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing. Store undiluted.

    储存条件

    4 °C,-20 °C

    储存方法

    4°C or -20°C, Avoid freeze-thaw cycles. Store undiluted.
  • 抗原

    CD40

    别名

    CD40

    背景

    Name/Gene ID: CD40
    Family: TNF Receptor

    Synonyms: CD40, B-cell surface antigen CD40, B cell-associated molecule, B cell surface antigen CD40, CD40 antigen, CD40 type II isoform, p50, Bp50, CD40L receptor, CDW40, TNFRSF5

    基因ID

    958

    UniProt

    P25942

    途径

    NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
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