Thrombospondin 1 抗体 (Collagen-binding Domain)
Quick Overview for Thrombospondin 1 抗体 (Collagen-binding Domain) (ABIN337292)
抗原
See all Thrombospondin 1 (THBS1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- Collagen-binding Domain
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品牌
- IHC-plus™
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特异性
- Recognizes Thrombospondin. Shows no cross-reactivity with fibronectin, fibrinogen and von Willebrand factor. Shows a mild cross-reaction with TSP 2. Inhibits TSP-collagen interaction. Antibody binding to TSP is unaffected by glycosaminoglycans (e. g. hyaluronic acid, chondroitin sulfate and heparin). Its binding is enhanced by EDTA i. e. at low concentration of Ca2+. Species cross-reactivity: Human, bovine, porcine, equine, canine, sheep, mouse and rat.
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纯化方法
- Protein G purified
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免疫原
- Reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets. MW of Antigen: ~450kD (non-reduced), 170-180kD (reduced). Epitope: Collagen Type V-binding domain of TSP.
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亚型
- IgG1
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anti-Thrombospondin 1 (THBS1) (AA 80-360) antibody
THBS1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Thrombospondin 1 (THBS1) (AA 515-671) antibodyTHBS1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Thrombospondin 1 (THBS1) (N-Term) antibodyVerified THBS1 适用: 人, 小鼠 WB, IHC, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Thrombospondin 1 (THBS1) (AA 24-221) antibodyTHBS1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Thrombospondin 1 (THBS1) (AA 71-259) antibodyTHBS1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Thrombospondin 1 (THBS1) (AA 750-850) antibodyTHBS1 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 5E2E2 unconjugated
anti-Thrombospondin 1 (THBS1) (AA 61-259) antibodyTHBS1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Thrombospondin 1 (THBS1) antibodyTHBS1 适用: 小鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Thrombospondin 1 (THBS1) (AA 1-1170) antibodyTHBS1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Thrombospondin 1 (THBS1) antibodyTHBS1 适用: 人, 小鼠 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Approved: Flo, IF, IHC, IHC-P (10 μg/mL), IP, WB
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, pH 7.4.
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储存液
- Azide free
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注意事项
- Avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Thrombospondin 1 (THBS1)
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别名
- Thrombospondin 1
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途径
- Autophagy
抗原
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