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Thrombospondin 1 抗体 (Collagen-binding Domain)

This 小鼠 单克隆 anti-Thrombospondin 1 antibody (Clone A6-1) specifically detects Thrombospondin 1 in WB, IHC, IP, IF, FACS 和 IHC (p). The antibody is reactive with 人, 小鼠, 大鼠, Cow, 马, Pig, 犬 和 绵羊 samples.
产品编号 ABIN337292
发货至: 中国
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Quick Overview for Thrombospondin 1 抗体 (Collagen-binding Domain) (ABIN337292)

抗原

See all Thrombospondin 1 (THBS1) 抗体
Thrombospondin 1 (THBS1)

适用

  • 62
  • 37
  • 19
  • 5
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
人, 小鼠, 大鼠, Cow, 马, Pig, 犬, 绵羊

宿主

  • 85
  • 8
  • 2
小鼠

克隆类型

  • 76
  • 19
单克隆

标记

  • 47
  • 18
  • 8
  • 5
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
This Thrombospondin 1 antibody is un-conjugated

应用范围

  • 63
  • 41
  • 29
  • 19
  • 13
  • 11
  • 10
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

A6-1
  • 抗原表位

    • 10
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Collagen-binding Domain

    特异性

    Recognizes Thrombospondin. Shows no cross-reactivity with fibronectin, fibrinogen and von Willebrand factor. Shows a mild cross-reaction with TSP 2. Inhibits TSP-collagen interaction. Antibody binding to TSP is unaffected by glycosaminoglycans (e. g. hyaluronic acid, chondroitin sulfate and heparin). Its binding is enhanced by EDTA i. e. at low concentration of Ca2+. Species cross-reactivity: Human, bovine, porcine, equine, canine, sheep, mouse and rat.

    纯化方法

    Protein G purified

    免疫原

    Reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets. MW of Antigen: ~450kD (non-reduced), 170-180kD (reduced). Epitope: Collagen Type V-binding domain of TSP.

    亚型

    IgG1
  • 应用备注

    Approved: Flo, IF, IHC, IHC-P (10 μg/mL), IP, WB

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS,  pH 7.4.

    储存液

    Azide free

    注意事项

    Avoid repeat freeze-thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • 抗原

    Thrombospondin 1 (THBS1)

    别名

    Thrombospondin 1

    途径

    Autophagy
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