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PRAS40 抗体

This 小鼠 单克隆 anti-PRAS40 antibody specifically detects PRAS40 in WB, IHC, IP 和 IHC (p). The antibody is reactive with 人 samples.
产品编号 ABIN337263
发货至: 中国
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中国
北京 101111
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Room 801-803
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Quick Overview for PRAS40 抗体 (ABIN337263)

抗原

See all PRAS40 (AKT1S1) 抗体
PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This PRAS40 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • 特异性

    Recognizes human PRAS40. Reacts with a 40kD band. This band is absent from PRAS40 siRNA-treated HeLa cell extracts. Does not cross-react with mouse.

    纯化方法

    Protein A/G purified

    免疫原

    Recombinant full length human PRAS40 expressed in E. coli.

    Type of Immunogen: Recombinant protein

    亚型

    IgG1 kappa
  • 应用备注

    Approved: IHC, IHC-P (10 μg/mL), IP, WB (0.1 - 1 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Suitable for use in Western Blot and immunoprecipitation.: Western Blot: 0.1-1 μg/mL. Positive control: HeLa cells, Jurkat cells.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS,  pH 7.2, 0.1 % sodium azide, 1 % BSA.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Short term: 4°C
    Long term: Add glycerol (40-50%) -20°C.
  • 抗原

    PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))

    别名

    AKT1S1 / PRAS40

    背景

    Name/Gene ID: AKT1S1

    Synonyms: AKT1S1, PRAS40, Proline-rich AKT1 substrate 1, Lobe

    基因ID

    84335

    UniProt

    Q96B36

    途径

    Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Cell Size, Autophagy, BCR Signaling, Warburg Effect
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