EZH2 抗体 (Internal Region)
Our Local Distributor
北京 101111
Quick Overview for EZH2 抗体 (Internal Region) (ABIN337251)
抗原
See all EZH2 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- Internal Region
-
特异性
- Recognizes the human EZH2 protein. On Western blots, it identifies bands at ~100kD and ~125kD, which may represent different isoforms of the protein. Reactivity has been confirmed with human Jurkat T cell leukemia, PC-3 prostate adenocarcinoma, BC-1 B cell lymphoma, Nalm-6 pre-B lymphocyte and HeLa cervical adenocarcinoma cell lysates by Western Blot and with formalin-fixed, paraffin-embedded (FFPE) human breast cancer, lymphoma, and normal prostate, esophagus, pancreas, stomach, liver, and brain tissues by immunohistochemistry. Species sequence homology: Mouse.
-
纯化方法
- Immunoaffinity purified
-
免疫原
-
Synthetic peptide derived from the internal region of the human EZH2 (ENX1) protein.
Type of Immunogen: Synthetic peptide -
亚型
- IgG
-
-
-
-
应用备注
-
Approved: ELISA (0.1 - 1 μg/mL), IHC, IHC-P (5 μg/mL), WB (0.5 - 2 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 0.5-2 μg/mL. Bands at ~100kD and ~125kD. -
说明
-
Target Species of Antibody: Human
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- Lot specific
-
缓冲液
- PBS, pH 7.4, 0.09 % sodium azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Avoid freeze-thaw cycles.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
-
-
- EZH2 (Enhancer of Zeste Homolog 2 (EZH2))
-
别名
- EZH2
-
背景
-
Name/Gene ID: EZH2
Synonyms: EZH2, ENX1, Enhancer of zeste homolog 2, KMT6, KMT6A, Lysine N-methyltransferase 6, WVS2, Enhancer of zeste, ENX-1 -
基因ID
- 2146
-
UniProt
- Q15910
-
途径
- Retinoic Acid Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation
抗原
-