CDK2 抗体
Quick Overview for CDK2 抗体 (ABIN337208)
抗原
See all CDK2 抗体适用
宿主
克隆类型
标记
应用范围
-
-
特异性
- Recognizes human Cdk2. Species cross-reactivity: mouse and rat.
-
纯化方法
- Protein A purified
-
免疫原
-
Human recombinant cdk2 protein.
Type of Immunogen: Recombinant protein -
亚型
- IgG2a, IgG2b
-
-
-
-
应用备注
-
Approved: ELISA, IF, IHC, IHC-P (5 μg/mL), IP, WB (1 - 2 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Immunoprecipitation: 2 μg/mg protein lysate. (Denatured only) Use Protein A. Western Blot: 1-2 μg/mL for 2hrs at RT. Positive control: HeLa cells. -
说明
-
Target Species of Antibody: Human
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- Lot specific
-
缓冲液
- 10 mM PBS, pH 7.4, 0.2 % BSA, 0.09 % sodium azide, 40 % glycerol.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Avoid repeated freezing and thawing.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- 4°C or -20°C, Avoid freeze-thaw cycles.
-
-
- CDK2 (Cyclin-Dependent Kinase 2 (CDK2))
-
别名
- CDK2
-
背景
-
Name/Gene ID: CDK2
Subfamily: CDC2/CDK
Family: Protein Kinase
Synonyms: CDK2, Cell division protein kinase 2, CDC2-related protein kinase, p33 protein kinase, p33(CDK2), CDKN2, Cyclin-dependent kinase 2 -
基因ID
- 1017
-
UniProt
- P24941
-
途径
- PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA
抗原
-