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Cullin 1 抗体 (C-Term)

This 小鼠 单克隆 anti-Cullin 1 antibody specifically detects Cullin 1 in WB, IHC, ELISA, IP 和 IHC (p). The antibody is reactive with 人 samples.
产品编号 ABIN337174
发货至: 中国
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Quick Overview for Cullin 1 抗体 (C-Term) (ABIN337174)

抗原

See all Cullin 1 (CUL1) 抗体
Cullin 1 (CUL1)

适用

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宿主

  • 93
小鼠

克隆类型

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单克隆

标记

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This Cullin 1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • 抗原表位

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    C-Term

    特异性

    Recognizes Cullin 1. Reacts specifically with the ~86kD CUL-1 protein and does not cross-react with the related CUL-2 protein. This antibody is confirmed to be reactive with human. Reactivity is confirmed from extracts of IMR90 (human fibroblasts) and Wi-38 cells.

    纯化方法

    Protein A purified

    免疫原

    A synthetic peptide corresponding to an 11 amino acid sequence at the C-terminus of the human CUL-1 protein.

    Type of Immunogen: Synthetic peptide

    亚型

    IgG1 kappa
  • 应用备注

    Approved: ELISA (0.1 - 1 μg/mL), IHC, IHC-P (10 μg/mL), IP, WB (1 - 3 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Immunoprecipitation: 5-10 μg/ IP reaction.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS,  pH 7.4, 0.1 % sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeat freeze-thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • 抗原

    Cullin 1 (CUL1)

    别名

    Cullin 1 / CUL1

    背景

    Name/Gene ID: CUL1

    Synonyms: CUL1, Cullin 1, Cullin-1, CUL-1

    基因ID

    8454

    UniProt

    Q13616

    途径

    Cell Division Cycle, Hedgehog Signaling, Mitotic G1-G1/S Phases, Regulation of Cell Size
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