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PINK1 抗体 (Internal Region)

This anti-PINK1 antibody is a 山羊 多克隆 antibody detecting PINK1 in WB, ELISA 和 FACS. Suitable for 人. This Primary Antibody has been cited in 1 publication.
产品编号 ABIN334460
发货至: 中国

Quick Overview for PINK1 抗体 (Internal Region) (ABIN334460)

抗原

See all PINK1 抗体
PINK1 (PTEN Induced Putative Kinase 1 (PINK1))

适用

  • 89
  • 29
  • 10
  • 2
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  • 1
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  • 1

宿主

  • 58
  • 44
山羊

克隆类型

  • 61
  • 43
多克隆

标记

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This PINK1 antibody is un-conjugated

应用范围

  • 77
  • 62
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  • 28
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Western Blotting (WB), ELISA, Flow Cytometry (FACS)

质量等级

Verified
  • 抗原表位

    • 28
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    • 2
    • 2
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    Internal Region

    原理

    PINK1

    序列

    QGKAHLESRS YQEAQ

    交叉反应

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-QGKAHLESRSYQEAQ, from the internal region of the protein sequence according to NP_115785.1.

    亚型

    IgG
  • 应用备注

    Western Blot: Approx. 60-65 kDa band observed in lysates of cell lines Jurkat and HeLa (calculated MW of 62.8 kDa according to NP_115785.1). Recommended concentration: 1-2 μg/mL. Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:16000.

    说明

    Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Choubey, Cagalinec, Liiv, Safiulina, Hickey, Kuum, Liiv, Anwar, Eskelinen, Kaasik: "BECN1 is involved in the initiation of mitophagy: it facilitates PARK2 translocation to mitochondria." in: Autophagy, Vol. 10, Issue 6, pp. 1105-19, (2014) (PubMed).

  • 抗原

    PINK1 (PTEN Induced Putative Kinase 1 (PINK1))

    别名

    PINK1

    背景

    PINK1, PTEN induced putative kinase 1 , BRPK, FLJ27236, PARK6 , protein kinase BRPK, serine/threonine-protein kinase PINK1

    基因ID

    65018, 68943, 298575

    NCBI登录号

    NP_115785

    途径

    Autophagy
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