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Patched 1 抗体 (Internal Region)

Cited in 1 publication. This 山羊 多克隆 anti-Patched 1 antibody specifically detects Patched 1 in ELISA 和 IF. The antibody is reactive with 斑马鱼 samples.
产品编号 ABIN334456
发货至: 中国
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Quick Overview for Patched 1 抗体 (Internal Region) (ABIN334456)

抗原

See all Patched 1 (PTCH1) 抗体
Patched 1 (PTCH1)

适用

  • 45
  • 29
  • 10
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
斑马鱼

宿主

  • 39
  • 7
  • 3
  • 3
山羊

克隆类型

  • 43
  • 9
多克隆

标记

  • 40
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This Patched 1 antibody is un-conjugated

应用范围

  • 35
  • 23
  • 19
  • 11
  • 6
  • 6
  • 3
  • 1
ELISA, Immunofluorescence (IF)

质量等级

Verified
  • 抗原表位

    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region

    原理

    Patched 1 (zebrafish)

    序列

    QTGSKKEPFN YSQ

    交叉反应

    斑马鱼

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence C-QTGSKKEPFNYSQ, from the internal region of the protein sequence according to NP_571063.1.

    亚型

    IgG
  • 应用备注

    Peptide ELISA: antibody detection limit dilution 1:32000.

    说明

    Immunofluorescence: This product has been successfully used in IF studies of zebrafish tumors (Ju et al, Mol Cancer. 2009 Jun 25,8:40., PMID: 19555497). Recommended concentration: 1-3μg/ml.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Ju, Spitsbergen, Eden, Taylor, Chen: "Co-activation of hedgehog and AKT pathways promote tumorigenesis in zebrafish." in: Molecular cancer, Vol. 8, pp. 40, (2009) (PubMed).

  • 抗原

    Patched 1 (PTCH1)

    别名

    ptc1

    背景

    Ptc1, patched 1 , ptc-1, etID309849.2, patched1

    NCBI登录号

    NP_571063

    途径

    Hedgehog Signaling, Carbohydrate Homeostasis, Tube Formation
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