ITCH 抗体 (Internal Region)
Quick Overview for ITCH 抗体 (Internal Region) (ABIN334426)
抗原
See all ITCH 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- Internal Region
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原理
- ITCH / AIF4
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序列
- EIKSHDLKPN GGN
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交叉反应
- Cow, 犬, 人, 小鼠, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-EIKSHDLKPNGGN, from the internal region of the protein sequence according to NP_113671.3.
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亚型
- IgG
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anti-Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH) antibody
KO Validated ITCH 适用: 人 WB 宿主: 兔 Monoclonal unconjugated
anti-Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH) (Tyr420) antibodyITCH 适用: 人 ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH) (AA 29-303) antibodyITCH 适用: 人 ELISA, WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH) (AA 92-190) antibodyITCH 适用: 人 ELISA, IF 宿主: 小鼠 Monoclonal 1B8 unconjugated
anti-Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH) (AA 50-150) antibodyKO Validated ITCH 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH) antibodyITCH 适用: 人 ELISA, WB 宿主: 兔 Monoclonal 9C3 unconjugated Recombinant Antibody
anti-Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH) (pTyr420) antibodyITCH 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH) (AA 143-192) antibodyITCH 适用: 人, Cow, 马, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH) (AA 410-437), (Middle Region) antibodyITCH 适用: 人, 小鼠, 大鼠, 猴 WB, FACS 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western Blot: Approx 90 kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 86.4 kDa according to NP_001244067.1) . Recommended concentration: 0.1-0.3 μg/mL. Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:16000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the nuclei of A431 and U2OS cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))
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别名
- ITCH
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背景
- ITCH, AIF4, itchy homolog E3 ubiquitin protein ligase (mouse) , AIP4, NAPP1, dJ468O1.1 , NFE2-associated polypeptide 1, atrophin-1 interacting protein 4, dJ468O1.1 (atrophin 1 interacting protein 4, AIP4), itchy homolog E3 ubiquitin protein ligase, ubiqui
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基因ID
- 83737, 16396, 311567
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NCBI登录号
- NP_113671, NP_001244066, NP_001244067
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途径
- Activation of Innate immune Response, CXCR4-mediated Signaling Events
抗原
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