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BHMT 抗体 (C-Term)

This anti-BHMT antibody is a 山羊 多克隆 antibody detecting BHMT in WB, IHC 和 ELISA. Suitable for 人, 小鼠 和 大鼠. This Primary Antibody has been cited in 4+ publications.
产品编号 ABIN334358
发货至: 中国

Quick Overview for BHMT 抗体 (C-Term) (ABIN334358)

抗原

See all BHMT 抗体
BHMT (Betaine--Homocysteine S-Methyltransferase (BHMT))

适用

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人, 小鼠, 大鼠

宿主

  • 29
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山羊

克隆类型

  • 29
  • 13
多克隆

标记

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This BHMT antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA

质量等级

Verified
  • 抗原表位

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    C-Term

    原理

    BHMT

    序列

    EQQLKELFEK QK

    交叉反应

    犬, 人, 小鼠, 大鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    免疫原

    C-EQQLKELFEKQK

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: Paraffin embedded Human Liver and Kidney. Recommended concentration: 5 μg/mL.

    Western Blot: Approx 45 kDa band observed in Rat Liver lysates (calculated MW of 44.9 kDa according to human NP_001704.1, 45.0 kDa according to mouse NP_057877.1 and rat NP_110477.1). This product has been successfully used in WB on Human (PMID: 27320863).

    Peptide ELISA: antibody detection limit dilution 1:16000.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Kim, Kwon, Kim: "Alterations in the metabolomics of sulfur-containing substances in rat kidney by betaine." in: Amino acids, Vol. 46, Issue 4, pp. 963-8, (2014) (PubMed).

    Yun, Ryu, Lee, Noh, Lee, Lee, Kang, Park, Kim, Kim: "Hepatic metabolism of sulfur amino acids in db/db mice." in: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, Vol. 53, pp. 180-6, (2013) (PubMed).

    Yun, Ryu, Oh, Kim, Lee, Lee, Lee, Kim, Kim: "Plasma homocysteine level and hepatic sulfur amino acid metabolism in mice fed a high-fat diet." in: European journal of nutrition, Vol. 52, Issue 1, pp. 127-34, (2013) (PubMed).

    Ryu, Kwak, Lee, Kang, Oh, Lee, Kim, Ma, Kim: "Sulfur amino acid metabolism in doxorubicin-resistant breast cancer cells." in: Toxicology and applied pharmacology, Vol. 255, Issue 1, pp. 94-102, (2011) (PubMed).

  • 抗原

    BHMT (Betaine--Homocysteine S-Methyltransferase (BHMT))

    别名

    BHMT

    背景

    BHMT, betaine-homocysteine methyltransferase

    基因ID

    635, 12116, 81508

    NCBI登录号

    NP_001704

    途径

    Methionine Biosynthetic Process
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