PODXL 抗体 (Extracellular Domain)
Quick Overview for PODXL 抗体 (Extracellular Domain) (ABIN325654)
抗原
See all PODXL 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Extracellular Domain
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特异性
- Recognizes human Podocalyxin. In Western Blots, this antibody does not cross-react with rmPodocalyxin or rhEndoglycan.
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纯化方法
- Protein G purified
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免疫原
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NS0-derived rhPodocalyxin extracellular domain.
Type of Immunogen: Recombinant protein -
亚型
- IgG2a
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应用备注
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Approved: Flo, ICC (10 μg/mL), WB
Usage: Suitable for use in Flow Cytometry, Immunocytochemistry and Western Blot. Immunocytochemistry: 10 μg/mL using fixed cells. Flow Cytometry: Dilute this antibody to 0.1 mg/mL and add 5 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 μL. Antibody binding may be visualized by adding 10 μL of a 25 μg/mL stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. Western Blot: t 1-2 μg/mL. Using a colorimetric detection system, the detection limit for rhPodocalyxin is approximately 5 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- 200 μL sterile PBS
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浓度
- Lot specific
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缓冲液
- Lyophilized from PBS, pH 7.4, 5 % trehalose.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
- 4°C or -20°C, Avoid freeze-thaw cycles.
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- PODXL (Podocalyxin-Like (PODXL))
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别名
- PODXL / Podocalyxin
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背景
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Name/Gene ID: PODXL
Synonyms: PODXL, GCTM-2 antigen, gp200, PCLP, PCLP-1, PCLP1, Podocalyxin-like protein 1, Podocalyxin, Podocalyxin-like, TRA-1-60, TRA-1-81 -
基因ID
- 5420
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UniProt
- O00592
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途径
- Tube Formation
抗原
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