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PODXL 抗体 (Extracellular Domain)

This 小鼠 单克隆 anti-PODXL antibody specifically detects PODXL in WB, FACS 和 ICC. The antibody is reactive with 人 samples.
产品编号 ABIN325654
发货至: 中国
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Quick Overview for PODXL 抗体 (Extracellular Domain) (ABIN325654)

抗原

See all PODXL 抗体
PODXL (Podocalyxin-Like (PODXL))

适用

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宿主

  • 74
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小鼠

克隆类型

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单克隆

标记

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This PODXL antibody is un-conjugated

应用范围

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Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC)
  • 抗原表位

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    Extracellular Domain

    特异性

    Recognizes human Podocalyxin. In Western Blots, this antibody does not cross-react with rmPodocalyxin or rhEndoglycan.

    纯化方法

    Protein G purified

    免疫原

    NS0-derived rhPodocalyxin extracellular domain.

    Type of Immunogen: Recombinant protein

    亚型

    IgG2a
  • 应用备注

    Approved: Flo, ICC (10 μg/mL), WB

    Usage: Suitable for use in Flow Cytometry, Immunocytochemistry and Western Blot. Immunocytochemistry: 10 μg/mL using fixed cells. Flow Cytometry: Dilute this antibody to 0.1 mg/mL and add 5 μL of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200 μL. Antibody binding may be visualized by adding 10 μL of a 25 μg/mL stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. Western Blot: t 1-2 μg/mL. Using a colorimetric detection system, the detection limit for rhPodocalyxin is approximately 5 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    200 μL sterile PBS

    浓度

    Lot specific

    缓冲液

    Lyophilized from PBS,  pH 7.4, 5 % trehalose.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C,-20 °C

    储存方法

    4°C or -20°C, Avoid freeze-thaw cycles.
  • 抗原

    PODXL (Podocalyxin-Like (PODXL))

    别名

    PODXL / Podocalyxin

    背景

    Name/Gene ID: PODXL

    Synonyms: PODXL, GCTM-2 antigen, gp200, PCLP, PCLP-1, PCLP1, Podocalyxin-like protein 1, Podocalyxin, Podocalyxin-like, TRA-1-60, TRA-1-81

    基因ID

    5420

    UniProt

    O00592

    途径

    Tube Formation
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