CD73 抗体 (AA 29-549)
Quick Overview for CD73 抗体 (AA 29-549) (ABIN324847)
抗原
See all CD73 (NT5E) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 29-549
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特异性
- Recognizes mouse CD73.
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纯化方法
- Immunoaffinity purified
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免疫原
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CHO cell-derived rmCD73. (aa 29 - 549).
Type of Immunogen: Recombinant protein -
亚型
- IgG
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应用备注
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Approved: ELISA (0.5 - 1 μg/mL), Flo, WB (0.1 - 0.2 μg/mL)
Usage: Suitable for use in Direct ELISA, Western Blot. and Flow Cytometry. Direct ELISA: 0.5-1.0 μg/mL. Detection limit for rmCD73 is. ~0.3 ng/well. Western Blot: 0.1-0.2 μg/mL. Detection limit for rmCD73 is ~1 ng/lane under non-reducing and reducing conditions. Flow Cytometry (Murine splenocytes): 25 μg/mL. Add 100 μL of the diluted solution to 1-2.5x10^5 cells in a total reaction volume not exceeding 200 μL. -
说明
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Target Species of Antibody: Mouse
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- PBS, 40-50 % glycerol
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浓度
- Lot specific
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缓冲液
- Lyophilized from PBS, 5 % trehalose.
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注意事项
- Aviod frequent freeze thaws.
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储存条件
- -20 °C
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储存方法
- Store at -20°C prior to reconstitution. After reconstitution aliquot and freeze at-20°C. Avoid freeze-thaw cycles.
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- CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))
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别名
- NT5E / eNT / CD73
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背景
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Name/Gene ID: NT5E
Synonyms: NT5E, 5-nucleotidase, ecto (CD73), 5'-nucleotidase, 5 nucleotidase (CD73), 5-NT, 5-nucleotidase, 5'-nucleotidase, ecto (CD73), CD73, CD73 antigen, E5NT, Ecto-5-nucleotidase, ENT, EN, NT, Purine 5-Prime-Nucleotidase, NT5, NTE -
基因ID
- 4907
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UniProt
- P21589
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途径
- Synaptic Membrane, Ribonucleoside Biosynthetic Process
抗原
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