Notch1 抗体 (AA 2428-2556)
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Quick Overview for Notch1 抗体 (AA 2428-2556) (ABIN324719)
抗原
See all Notch1 (NOTCH1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 2428-2556
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特异性
- Recognizes human Notch-1 IC.
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纯化方法
- Protein A/G purified
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免疫原
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E. coli-derived rhNotch-1 IC. (aa 2428 - 2556).
Type of Immunogen: Recombinant protein -
亚型
- IgG2a
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anti-Notch 1 (NOTCH1) antibody (APC)
NOTCH1 适用: 人, 小鼠 ICFC 宿主: 小鼠 Monoclonal mN1A APC
anti-Notch 1 (NOTCH1) antibody (PE)NOTCH1 适用: 人, 小鼠 ICFC 宿主: 小鼠 Monoclonal mN1A PE
anti-Notch 1 (NOTCH1) (AA 1919-2079) antibodyNOTCH1 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Notch 1 (NOTCH1) (AA 2101-2300) antibodyNOTCH1 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Notch 1 (NOTCH1) (Cleaved-Val1754) antibodyNOTCH1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Notch 1 (NOTCH1) antibodyKD Validated NOTCH1 适用: 人 WB, FACS 宿主: 兔 Monoclonal 25GB425 unconjugated Recombinant Antibody
anti-Notch 1 (NOTCH1) (AA 20-216) antibodyNOTCH1 适用: 小鼠 WB, ICC, IF 宿主: 小鼠 Monoclonal S253-32 unconjugated
anti-Notch 1 (NOTCH1) (AA 20-216) antibody (HRP)NOTCH1 适用: 小鼠 WB, ICC, IF 宿主: 小鼠 Monoclonal S253-32 HRP
anti-Notch 1 (NOTCH1) (AA 20-216) antibody (FITC)NOTCH1 适用: 小鼠 WB, ICC, IF 宿主: 小鼠 Monoclonal S253-32 FITC
anti-Notch 1 (NOTCH1) (AA 20-216) antibody (Biotin)NOTCH1 适用: 小鼠 WB, ICC, IF 宿主: 小鼠 Monoclonal S253-32 Biotin
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应用备注
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Approved: ELISA (0.5 - 1 μg/mL), ICC (10 μg/mL), WB (1 - 2 μg/mL)
Usage: Suitable for use in Direct ELISA, Western Blot and Immunocytochemistry. Direct ELISA: 0.5-1.0 μg/mL detection limit for rhNotch-1 IC is ~3 ng/well. Western Blot: 1-2 μg/mL detection limit for rhNotch-1 IC is ~25 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 10 μg/mL. For SAOS2 osteosarcoma cells, cells were fixed with PBS containing 4 % paraformaldehyde for 20 min at RT and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100 and 1 % BSA for 45 min at RT. After blocking, cells were incubated with primary antibody overnight at 4?C followed by fluorochrome-coupled anti-mouse IgG at RT in the dark for 1 hour. Between each step, cells were washed with PBS containing 0.1 % BSA. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Sterile PBS
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浓度
- Lot specific
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缓冲液
- Lyophilized, PBS, 5 % trehalose
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注意事项
- Avoid freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Notch1 (NOTCH1) (Notch 1 (NOTCH1))
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别名
- NOTCH1
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背景
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Name/Gene ID: NOTCH1
Synonyms: NOTCH1, Notch 1, TAN1 -
基因ID
- 4851
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UniProt
- P46531
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途径
- Notch Signaling, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
抗原
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