Notch1 抗体 (AA 20-216)
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- 抗原 See all Notch1 (NOTCH1) 抗体
- Notch1 (NOTCH1) (Notch 1 (NOTCH1))
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抗原表位
- AA 20-216
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适用
- 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Notch1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
- 特异性
- Detects >270 kDa. Does not cross-react with Notch 2 or Notch3.
- 交叉反应
- 人, 小鼠, 大鼠
- 纯化方法
- Protein G Purified
- 免疫原
- Fusion protein amino acids 20-216 (extracellular N-terminus, EGF-like domains 1-5) of mouse Notch1
- 克隆位点
- S253-32
- 亚型
- IgG1
- Top Product
- Discover our top product NOTCH1 Primary Antibody
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anti-Notch 1 (NOTCH1) antibody (PE)
NOTCH1 适用: 人, 小鼠 FACS 宿主: 小鼠 Monoclonal mN1A PE
anti-Notch 1 (NOTCH1) antibody (APC)NOTCH1 适用: 人, 小鼠 FACS 宿主: 小鼠 Monoclonal mN1A APC
anti-Notch 1 (NOTCH1) (AA 2101-2300) antibodyNOTCH1 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Notch 1 (NOTCH1) (Cleaved-Val1754) antibodyNOTCH1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Notch 1 (NOTCH1) (AA 20-216) antibody (HRP)NOTCH1 适用: 小鼠 WB, IF, ICC 宿主: 小鼠 Monoclonal S253-32 HRP
anti-Notch 1 (NOTCH1) (AA 20-216) antibody (FITC)NOTCH1 适用: 小鼠 WB, IF, ICC 宿主: 小鼠 Monoclonal S253-32 FITC
anti-Notch 1 (NOTCH1) (AA 20-216) antibody (Biotin)NOTCH1 适用: 小鼠 WB, IF, ICC 宿主: 小鼠 Monoclonal S253-32 Biotin
anti-Notch 1 (NOTCH1) (AA 20-216) antibody (PE)NOTCH1 适用: 小鼠 WB, IF, ICC 宿主: 小鼠 Monoclonal S253-32 PE
anti-Notch 1 (NOTCH1) (AA 20-216) antibody (APC)NOTCH1 适用: 小鼠 WB, IF, ICC 宿主: 小鼠 Monoclonal S253-32 APC
anti-Notch 1 (NOTCH1) (AA 20-216) antibody (PerCP)NOTCH1 适用: 小鼠 WB, IF, ICC 宿主: 小鼠 Monoclonal S253-32 PerCP
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- 应用备注
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- WB (1:1000)
- optimal dilutions for assays should be determined by the user.
- 说明
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1 μg/ml of ABIN1686639 was sufficient for detection of Notch1 in 20 μg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- -20°C
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- 抗原
- Notch1 (NOTCH1) (Notch 1 (NOTCH1))
- 别名
- Notch1 (NOTCH1 产品)
- 别名
- notch1 antibody, NOTCH1 antibody, hn1 antibody, tan1 antibody, notch antibody, xotch antibody, xnotch antibody, notch-1 antibody, xnotch1 antibody, TAN1 antibody, hN1 antibody, 9930111A19Rik antibody, Mis6 antibody, N1 antibody, Tan1 antibody, lin-12 antibody, NOTCH antibody, Xotch antibody, notch1-a antibody, notch 1 antibody, fibropellin-1 antibody, Notch homolog 1, translocation-associated (Drosophila) antibody, Notch homolog 1, translocation-associated antibody, notch 1 S homeolog antibody, notch1 antibody, LOC100631526 antibody, NOTCH1 antibody, Notch1 antibody, notch1.S antibody
- 背景
- Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.
- 基因ID
- 18128
- NCBI登录号
- NP_032740
- UniProt
- Q01705
- 途径
- Notch Signaling, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
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