PC 抗体
(2 references)Our Local Distributor
北京 101111
Quick Overview for PC 抗体 (ABIN3197484)
抗原
适用
宿主
克隆类型
标记
应用范围
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原理
- Anti-PC | Plastocyanin
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交叉反应 （详细）
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Arabidops thaliana, Brassica juncea, Chlamydomonas reinhardtii, Heliantus annuus, Hordeum vulgare, Lathyrus sativus, Marchantia polymorpha, Nicotiana tabacum, Oryza sativa, Pisum sativum, Spinacia oleracea, Solanum tuberosum, Synechocystis sp. PCC6803, Zea mays
Not reactive in: No confirmed exceptions from predicted reactivity are currently known -
预测反应
- Dicots,Chlamydomonas reinhardtii,Nicotiana benthamiana,Physcomitrium patens,Ricinus communis,Solanum lycopersicum,Suaeda salsa
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纯化方法
- Serum
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免疫原
- Purified native plastocyanin from Spinacia oleracea UniProt: P00289
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anti-PC (AA 53-82), (N-Term) antibody
适用: 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB31105 unconjugated
anti-PC (AA 53-82), (N-Term) antibody适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-PC (C-Term) antibody适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-PC (AA 357-406) antibody适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
- 1 : 100 (IG), 1 : 2000 (WB)
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说明
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plastocyanin runs abberant due to negative charge at 12-19 kDa on SDS-PAGE depending upon the system used. in 15 % gel the protein will run closer to its true MW than in 12 % gel. In some cases PC can be very acidic and run at twice of its MW.PC1 runs closer to 14 kDa while PC2 runs closer to 19 kDa. For good resolution adding fresh DTT to the sample buffer is recommended. PC2 is generally more abundant and it increases with Cu feeding. PC1 is expressed first after etiolated seedlings are placed in the light.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 50 μL of sterile water
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缓冲液
- Serum
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注意事项
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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储存条件
- -20 °C
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储存方法
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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: "Arabidopsis copper transport protein COPT2 participates in the cross talk between iron deficiency responses and low-phosphate signaling." in: Plant physiology, Vol. 162, Issue 1, pp. 180-94, (2013) (PubMed).
: "Protocol: Optimised methodology for isolation of nuclei from leaves of species in the Solanaceae and Rosaceae families." in: Plant methods, Vol. 9, Issue 1, pp. 31, (2013) (PubMed).
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- PC
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背景
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Background: Plastocyanin (PC) is a small Cu protein and a mobile electron carrier in the lumen of the thylkoids. PC interacts with the B/F complex and Photosystem I. Alternative name: DNA-damage-repair/toleration protein DRT112.
Additional information: Cellular [compartment marker] of chloroplast thylakoid lumenThis product can be sold containing ProClin if requested.
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分子量
- 10 kDa
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基因ID
- 838622
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UniProt
- P00289
抗原
-
