PC 抗体
(2 references)-
- 抗原
- PC
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适用
- Nicotiana tabacum, Oryza sativa, Pisum sativum, Potato, Spinach, Wild Cabbage, Zea mays
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- 非结合性
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应用范围
- Western Blotting (WB), Electron Microscopy (EM)
- 产品特性
- Expected / apparent Molecular Weight of the Antigene: 10 kDa
- 纯化方法
- serum
- 免疫原
- purified native plastocyanin from Spinacia oleracea P00289
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anti-PC (AA 53-82), (N-Term) antibody
适用: 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB31105 unconjugated
anti-PC (AA 53-82), (N-Term) antibody适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-PC antibody适用: 人, 小鼠, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-PC (AA 357-406) antibody适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-PC (C-Term) antibody适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, Pig WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Recommended Dilution: 1 : 2000 with alkaline phosphatase (WB), 1 : 100 (IG) MW 10 kDa.
Cellular [compartment marker] of chloroplast thylakoid lumen - 说明
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plastocyanin runs abberant due to negative charge at 12-19 kDa on SDS-PAGE depending upon the system used. in 15 % gel the protein will run closer to its true MW than in 12 % gel. In some cases PC can be very acidic and run at twice of its MW.PC1 runs closer to 14 kDa while PC2 runs closer to 19 kDa. For good resolution adding fresh DTT to the sample buffer is recommended. PC2 is generally more abundant and it increases with Cu feeding. PC1 is expressed first after etiolated seedlings are placed in the light.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- For reconstitution add 10 µL of sterile water.
- 注意事项
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Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. - 储存条件
- -20 °C
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-
: "Arabidopsis copper transport protein COPT2 participates in the cross talk between iron deficiency responses and low-phosphate signaling." in: Plant physiology, Vol. 162, Issue 1, pp. 180-94, (2013) (PubMed).
: "Protocol: Optimised methodology for isolation of nuclei from leaves of species in the Solanaceae and Rosaceae families." in: Plant methods, Vol. 9, Issue 1, pp. 31, (2013) (PubMed).
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: "Arabidopsis copper transport protein COPT2 participates in the cross talk between iron deficiency responses and low-phosphate signaling." in: Plant physiology, Vol. 162, Issue 1, pp. 180-94, (2013) (PubMed).
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- 抗原
- PC
- 背景
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AGI Code: At1g20340
Plastocyanin (PC) is a small Cu protein and a mobile electron carrier in the lumen of the thylkoids. PC interacts with the B/F complex and Photosystem I. Alternative name: DNA-damage-repair/toleration protein DRT112. - 分子量
- 10 kDa
- UniProt
- P00289
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