Integrin beta 3 抗体
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- 抗原 See all Integrin beta 3 (ITGB3) 抗体
- Integrin beta 3 (ITGB3)
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Integrin beta 3 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 纯化方法
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- 免疫原
- PHA stimulated peripheral blood cells. Spleen cells from immunised Balb/c mice were fused with cells of the NS1 myeloma cell line.
- 克隆位点
- Y2-51
- 亚型
- IgG1
- Top Product
- Discover our top product ITGB3 Primary Antibody
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- 应用备注
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Flow Cytometry: use 10 μL of 1/100-1/200 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on Frozen and Paraffin Sections: This product requires proteindigestion pre-treatment of paraffin sections e. g. trypsin prior to staining. Western Blotting: Detects a band/s of approximately 90 kDa in platelet lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- Integrin beta 3 (ITGB3)
- 别名
- CD61 / ITGB3 (ITGB3 产品)
- 背景
- CD61 is a 105kD glycoprotein, which is also known as integrin beta 3 chain. This molecule associates with either the alpha IIb integrin (CD41) or the alpha V integrin (CD51) at the cell surface. CD61 is expressed on platelets and megakaryocytes in association with CD41, and on endothelial cells, monocytes, platelets and osteoclasts in association with CD51. CD61 is a receptor for fibrinogen, fibronectin, vWF, vitronectin and thrombospondin.Synonyms: GP3A, GPIIIa, Integrin beta-3, Platelet membrane glycoprotein IIIa
- 基因ID
- 9606
- UniProt
- P05106
- 途径
- Regulation of G-Protein Coupled Receptor Protein Signaling, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Integrin Complex
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