SYK 抗体 (AA 321-325)
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Quick Overview for SYK 抗体 (AA 321-325) (ABIN319415)
抗原
See all SYK 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 321-325
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特异性
- This antibody detects endogenous levels of total Syk protein.
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纯化方法
- Immunoaffinity Chromatography using epitope-specific immunogen.
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免疫原
- Peptide sequenece around amino acids 321~325 (N-P-Y-E-P) derived from Human Syk.
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应用备注
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Western Blot: 1/500approx. 1/1000. Immunofluorescence: 1/100approx. 1/200. Immunohistochemistry on Paraffin Sections: 1/50approx. 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the antibody (in aliquots) at-20 °C.
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- SYK (Spleen tyrosine Kinase (SYK))
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别名
- SYK
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背景
- Syk (72 kDa) is a non receptor protein tyrosine kinase that plays an important role in immune receptor signal transduction and is implicated in endothelial cell functions, including cell growth and migration. SYK is a positive effector of BCR stimulated responses. It couples the B cell antigen receptor (BCR) to the mobilization of calcium ions either through a phosphoinositide 3 kinase dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C gamma dependent pathway, when phosphorylated on Tyr 342 and Tyr 346. Thus the differential phosphorylation of SYK can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ions.Synonyms: Spleen tyrosine kinase, Tyrosine-protein kinase SYK
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基因ID
- 6850
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NCBI登录号
- NP_001128524
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UniProt
- P43405
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途径
- Fc-epsilon Receptor Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Thromboxane A2 Receptor Signaling, BCR Signaling
抗原
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