Growth Hormone 1 抗体
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北京 101111
Quick Overview for Growth Hormone 1 抗体 (ABIN318574)
抗原
See all Growth Hormone 1 (GH1) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody recognizes human Growth Hormone (GH), which acts to control body growth and metabolic activity. In Western blotting a band of approximately 20 kDa is seen using lysates of human pituitary gland.
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纯化方法
- Purified IgG prepared by affinity chromatography on Protein G
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免疫原
- Growth hormone from ovine pituitary.
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亚型
- IgG
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应用备注
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Western blot: 1/50-1/500. Immunofluorescence: 1/10-1/100. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: 1/500-1/4000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 5.0 mg/mL
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缓冲液
- Phosphate buffered saline containing 0.09 % Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Storage in frost-free freezers is not recommended.
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- Growth Hormone 1 (GH1)
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别名
- Somatotropin / Growth Hormone / GH1
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背景
- Human growth hormone (hGH) plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. The effects of hGH in the body can be generally described as anabolic. It stimulates amino acid uptake and protein synthesis in muscle and other tissues. Growth Hormone (GH1) is synthesized by acidophilic or somatotropic cells of the anterior pituitary gland. Genes for growth hormone (GH) are found in a gene cluster on 17q, which consists of two growth hormone genes and three CSH genes. The GHN1 gene is transcribed exclusively in the pituitary, whereas the other 4 genes are expressed only in placental tissues. Several isoforms of GH1 exist. Human growth hormone (hGH) is also used as a reporter protein, because it is secreted from mammalian cells it can be assayed by ELISA on culture supernatant using antibodies to determine the level of expression.Synonyms: Growth hormone 1, HGH, Pituitary growth hormone
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基因ID
- 2688
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NCBI登录号
- NP_000506
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UniProt
- P01241
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途径
- NF-kappaB Signaling, JAK/STAT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Response to Growth Hormone Stimulus, Regulation of Hormone Biosynthetic Process
抗原
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