Cathepsin G 抗体 (Internal Region)
(1 validation)Quick Overview for Cathepsin G 抗体 (Internal Region) (ABIN3183694)
抗原
See all Cathepsin G (CTSG) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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特异性
- Cathepsin G Polyclonal Antibody detects endogenous levels of Cathepsin G protein.
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产品特性
- Rabbit Polyclonal to Cathepsin G.
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纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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免疫原
- Synthesized peptide derived from the Internal region of human Cathepsin G.
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亚型
- IgG
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anti-Cathepsin G (CTSG) (AA 21-255) antibody
CTSG 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Cathepsin G (CTSG) (AA 21-255) antibodyCTSG 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cathepsin G (CTSG) (AA 75-190) antibodyCTSG 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Cathepsin G (CTSG) (AA 21-261) antibodyCTSG 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cathepsin G (CTSG) (AA 1-255) antibodyCTSG 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Cathepsin G (CTSG) (Internal Region) antibodyCTSG 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cathepsin G (CTSG) (AA 51-250) antibodyCTSG 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cathepsin G (CTSG) (AA 1-255) antibodyCTSG 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Cathepsin G (CTSG) (AA 41-120) antibodyCTSG 适用: 人, 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cathepsin G (CTSG) (Cleaved-Ile21) antibodyCTSG 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
- WB 1:500-1:2000, ELISA 1:40000,
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限制
- 仅限研究用
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- by
- NanoTemper Technologies
- No.
- #102721
- 日期
- 2018.11.05
- 抗原
- CTSG
- Lot Number
- 022204892
- Method validated
- Unfolding Profile
- Positive Control
- ABIN3183694
- Negative Control
- Notes
Passed. ABIN3183694 showed Ti at 85.2°C and a clear unfolding profile with one unfolding event. This suggests that the protein is properly folded and functional.
- Primary Antibody
- ABIN3183694
- Secondary Antibody
- Full Protocol
- Dilute ABIN3183694 1:10 in PBS buffer (Roth, 1058.1, lot 285231988) to get a final volume of 15µl at a concentration of 0.67µM.
- Load sample into Tycho capillary (NanoTemper Technologies, TY-C001).
- Run Tycho measurement.
- Experimental Notes
Tycho is designed to run quick and precise protein quality check experiments. Tycho uses intrinsic protein fluorescence to follow protein unfolding while running a fast thermal ramp, yielding results in 3min. A protein’s unfolding behavior is characterized by various parameters, most notably the inflection temperature (Ti). The Ti can be used to identify properly folded protein, to compare different batches, or to analyze the influence of storage/transport conditions on a protein. An absence of Ti would suggest that the protein is already unfolded and therefore most likely nonfunctional.
生效 #102721 (Unfolding Profile)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![p>Unfolding profile of ABIN3183694. The fluorescence signal is plotted against temperature. The native (folded) protein has a high signal at the beginning of the experiment, which decreases and then again increases upon unfolding, showing one unfolding event. The vertical line indicates the Ti at 85.2°C.]()
p>Unfolding profile of ABIN3183694. The fluorescence signal is plotted against temperature. The native (folded) protein has a high signal at the beginning of the experiment, which decreases and then again increases upon unfolding, showing one unfolding event. The vertical line indicates the Ti at 85.2°C.
Full Methods -
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- -20 °C
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储存方法
- Store at -20°C.
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- Cathepsin G (CTSG)
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别名
- Cathepsin G
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分子量
- 22 kDa
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基因ID
- 1511
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UniProt
- P08311
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途径
- ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones
抗原
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