Focal Adhesion Kinase (FAK) is a 120 kDa tyrosine kinase which become phosphorylated in response to oncogene- and extracellular-matrix integrin-dependent stimulation. Chicken, mouse and human FAK has been isolated and the deduced amino acid sequences showed identity of over 90 % . It is expressed in embryonic stem cells, and ubiquitously throughout development, in all adult tissues examined and in many cell lines. The highest expression of FAK is seen in the brain, testes and in osteoclasts. It is distinct from other non-receptor protein tyrosine kinases in that its catalytic domain is flanked by amino- and carboxy-terminal domains of about 400 amino acids each. Also, it does not contain the Src homology 2 or 3 domains, which mediate protein-protein interaction, nor a myristylation site that anchors proteins to the membrane.
纯化方法
Ammonium Sulfate Precipitation
免疫原
Bacterially expressed fusion protein consisting of the C-terminal 150 amino acids of the mouse FAK
Detect focal adhesion kinase by IP/Western analysis in BALB/c 3T3 fibroblasts1. Detects a 120 kDa band. Optimal concentration should be evaluated by serial dilutions.
限制
仅限研究用
缓冲液
Provided as solution in phosphate buffered saline with 0.08 % sodium azide
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
-20 °C
储存方法
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
抗原
FAK (PTK2)
(PTK2 Protein tyrosine Kinase 2 (PTK2))