14-3-3 sigma/SFN 抗体 (Middle Region)
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北京 101111
Quick Overview for 14-3-3 sigma/SFN 抗体 (Middle Region) (ABIN3044182)
抗原
See all 14-3-3 sigma/SFN (SFN) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Middle Region
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原理
- Anti-14-3-3 sigma/SFN Antibody
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序列
- KKRIIDSARS AYQEAMD
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特异性
- No cross reactivity with other proteins.
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交叉反应 （详细）
- No cross-reactivity with other proteins
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预测反应
- identical to the related rat,mouse sequences.
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产品特性
- Anti-14-3-3 sigma/SFN Antibody. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence in the middle region of human 14-3-3 sigma, identical to the related rat and mouse sequences.
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亚型
- IgG
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anti-Stratifin (SFN) antibody
SFN 适用: 人 WB, IF, ICC, IP, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Stratifin (SFN) (Internal Region) antibodyVerified SFN 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Stratifin (SFN) (AA 1-248) antibodySFN 适用: 人 WB, ELISA, IP, IHC (p) 宿主: 小鼠 Monoclonal 3C3 unconjugated
anti-Stratifin (SFN) (C-Term) antibodySFN 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Stratifin (SFN) (AA 1-248) antibodySFN 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, FACS, ICC 宿主: 兔 Polyclonal unconjugated
anti-Stratifin (SFN) (AA 1-248) antibodySFN 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2G2 unconjugated
anti-Stratifin (SFN) (AA 1-249) antibodySFN 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 3C3 unconjugated
anti-Stratifin (SFN) antibodySFN 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal CPTC-SFN-2 unconjugated
anti-Stratifin (SFN) (AA 222-248), (C-Term) antibodySFN 适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB27523 unconjugated
anti-Stratifin (SFN) (AA 198-248) antibodySFN 适用: 人 IHC, IF (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
- Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human Immunocytochemistry , 0.5-1 μg/mL, Human Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
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说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. -
有效期
- 12 months
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: "Histone acetyltransferase inhibitor II induces apoptosis in glioma cell lines via the p53 signaling pathway." in: Journal of experimental & clinical cancer research : CR, Vol. 33, pp. 108, (2015) (PubMed).
: "Makorin-2 is a neurogenesis inhibitor downstream of phosphatidylinositol 3-kinase/Akt (PI3K/Akt) signal." in: The Journal of biological chemistry, Vol. 283, Issue 13, pp. 8486-95, (2008) (PubMed).
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- 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))
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别名
- SFN
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背景
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Background: Stratifin (SFN), also known as 14-3-3 protein sigma, is strongly induced by gamma irradiation and other DNA-damaging agents. The induction of 14-3-3-sigma is mediated by a p53 -responsive element located 1.8 kb upstream of its transcription start site. Leffers et al. (1993)obtained peptide sequence and subsequently cloned a T-cell cDNA of the 14-3-3 family of conserved proteins. The protein, called stratifin, was shown to be diffusely distributed in the cytoplasm and was present in cultured epithelial cells. It was most abundant in tissues enriched in stratified keratinizing epithelium.
Gene Full Name: stratifin
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分子量
- 28 kDa
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基因ID
- 2810
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UniProt
- P31947
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途径
- p53 Pathway, Myometrial Relaxation and Contraction
抗原
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