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14-3-3 sigma/SFN 抗体 (Middle Region)

SFN 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC, FACS 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN3044182
发货至: 中国
  • 抗原 See all 14-3-3 sigma/SFN (SFN) 抗体
    14-3-3 sigma/SFN (SFN) (Stratifin (SFN))
    抗原表位
    • 14
    • 14
    • 7
    • 7
    • 6
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    AA 140-156, Middle Region
    适用
    • 92
    • 38
    • 28
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    人, 小鼠, 大鼠
    宿主
    • 82
    • 9
    • 2
    • 1
    克隆类型
    • 76
    • 18
    多克隆
    标记
    • 43
    • 8
    • 6
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This 14-3-3 sigma/SFN antibody is un-conjugated
    应用范围
    • 69
    • 33
    • 32
    • 20
    • 15
    • 14
    • 13
    • 12
    • 11
    • 9
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)
    原理
    Anti-14-3-3 sigma/SFN Antibody Picoband®
    序列
    KKRIIDSARS AYQEAMD
    交叉反应 (详细)
    No cross-reactivity with other proteins
    产品特性
    Anti-14-3-3 sigma/SFN Antibody (ABIN3044182). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
    纯化方法
    Immunogen affinity purified.
    免疫原
    A synthetic peptide corresponding to a sequence in the middle region of human 14-3-3 sigma, identical to the related rat and mouse sequences.
    亚型
    IgG
    Top Product
    Discover our top product SFN Primary Antibody
  • 应用备注
    Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
    Immunocytochemistry , 0.5-1 μg/mL, Human
    Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human1. Leffers, H., Madsen, P., Rasmussen, H. H., Honore, B., Andersen, A. H., Walbum, E., Vandekerckhove, J., Celis, J. E.Molecular cloning and expression of the transformation sensitive epithelial marker stratifin: a member of a protein family that has been involved in the protein kinase C signalling pathway.J. Molec. Biol. 231: 982-998, 1993. 2. Urano, T., Saito, T., Tsukui, T., Fujita, M., Hosoi, T., Muramatsu, M., Ouchi, Y., Inoue, S. Efp targets 14-3-3-sigma for proteolysis and promotes breast tumour growth.Nature 417: 871-875, 2002. 3. Wilker, E. W., van Vugt, M. A. T. M., Artim, S. A., Huang, P. H., Petersen, C. P., Reinhardt, H. C., Feng, Y., Sharp, P. A., Sonenberg, N., White, F. M., Yaffe, M. B.14-3-3-sigma controls mitotic translation to facilitate cytokinesis.Nature 446: 329-332, 2007.
    说明

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    浓度
    500 μg/mL
    缓冲液
    Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    4 °C,-20 °C
    储存方法
    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
    有效期
    12 months
  • Xu, Li, Tao, Li, Fang, Zhao, Li, Li, Wang, Feng, Pan: "Histone acetyltransferase inhibitor II induces apoptosis in glioma cell lines via the p53 signaling pathway." in: Journal of experimental & clinical cancer research : CR, Vol. 33, pp. 108, (2015) (PubMed).

    Yang, Cheung, Peng, He, Wu, Xie, Jiang, Huang, Chen, Lin, Kung: "Makorin-2 is a neurogenesis inhibitor downstream of phosphatidylinositol 3-kinase/Akt (PI3K/Akt) signal." in: The Journal of biological chemistry, Vol. 283, Issue 13, pp. 8486-95, (2008) (PubMed).

  • 抗原
    14-3-3 sigma/SFN (SFN) (Stratifin (SFN))
    别名
    SFN (SFN 产品)
    背景

    Synonyms: 14-3-3 protein sigma,Epithelial cell marker protein 1,Stratifin,SFN,HME1,

    Tissue Specificity: Present mainly in tissues enriched in stratified squamous keratinizing epithelium.

    Background: Stratifin (SFN), also known as 14-3-3 protein sigma, is strongly induced by gamma irradiation and other DNA-damaging agents. The induction of 14-3-3-sigma is mediated by a p53 -responsive element located 1.8 kb upstream of its transcription start site. Leffers et al. (1993)obtained peptide sequence and subsequently cloned a T-cell cDNA of the 14-3-3 family of conserved proteins. The protein, called stratifin, was shown to be diffusely distributed in the cytoplasm and was present in cultured epithelial cells. It was most abundant in tissues enriched in stratified keratinizing epithelium.

    分子量
    28 kDa
    UniProt
    P31947
    途径
    p53 Pathway, Myometrial Relaxation and Contraction
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