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GLI3 抗体 (N-Term)

Name: GLI3 抗体 (N-Term)
SKU: ABIN3044156
Availability: OutOfStock

This anti-GLI3 antibody is a 兔多克隆 antibody detecting GLI3 in WB. Suitable for 人, 小鼠和大鼠. This Primary Antibody has been cited in 1 publication.

Anti-Gli3 antibody, Western blotting Lane 1: Rat Testis Tissue Lysate Lane 2: A549 Cell Lysate Lane 3: SW620 Cell Lysate (GLI3 抗体 (N-Term))

1 image

(1 reference)

产品编号 ABIN3044156

发货至: 中国

Datasheet as PDF

Quick Overview for GLI3 抗体 (N-Term) (ABIN3044156)

抗原

See all GLI3 抗体

GLI3 (GLI Family Zinc Finger 3 (GLI3))

适用

人, 小鼠, 大鼠

宿主

兔

克隆类型

多克隆

标记

This GLI3 antibody is un-conjugated

应用范围

Western Blotting (WB)

Discover our top product GLI3 Primary Antibody

抗原表位
5

4

3

3

1

1

1

1

1

1
AA 40-57, N-Term

原理

Anti-Transcriptional activator GLI3 Gli3 Antibody Picoband®

序列

SNEDESPGQT YHRERRNA

交叉反应（详细）

No cross-reactivity with other proteins

产品特性

Anti-Transcriptional activator GLI3 Gli3 Antibody (ABIN3044156). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

纯化方法

Immunogen affinity purified.

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human Gli3, different from the related rat sequence by two amino acids, and from the related mouse sequence by one amino acid.

亚型

IgG
anti-GLI Family Zinc Finger 3 (GLI3) antibody
GLI3 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-GLI Family Zinc Finger 3 (GLI3) (AA 1-110) antibody
GLI3 适用: 人 WB, ELISA, IF, PLA 宿主: 小鼠 Monoclonal 2C9 unconjugated

anti-GLI Family Zinc Finger 3 (GLI3) (AA 303-561) antibody
GLI3 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-GLI Family Zinc Finger 3 (GLI3) (AA 30-60) antibody
GLI3 适用: 人 WB, IHC, ELISA, IF, FM, MA 宿主: 兔 Polyclonal unconjugated

anti-GLI Family Zinc Finger 3 (GLI3) (AA 1-110) antibody
GLI3 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated

anti-GLI Family Zinc Finger 3 (GLI3) (AA 1-110) antibody
GLI3 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1H7 unconjugated

anti-GLI Family Zinc Finger 3 (GLI3) (AA 111-343) antibody
GLI3 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-GLI Family Zinc Finger 3 (GLI3) (AA 800-900) antibody
GLI3 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-GLI Family Zinc Finger 3 (GLI3) (AA 11-60) antibody
GLI3 适用: 人, 小鼠, 大鼠 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-GLI Family Zinc Finger 3 (GLI3) (N-Term) antibody
GLI3 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated

应用备注

Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
1. Barna, M., Pandolfi, P. P., Niswander, L. Gli3 and Plzf cooperate in proximal limb patterning at early stages of limb development. (Letter) Nature 436: 277-281, 2005. 2. Bose, J., Grotewold, L., Ruther, U. Pallister-Hall syndrome phenotype in mice mutant for Gli3. Hum. Molec. Genet. 11: 1129-1135, 2002. 3. Craig, D. W., Itty, A., Panganiban, C., Szelinger, S., Kruer, M. C., Sekar, A., Reiman, D., Narayanan, V., Stephan, D. A., Kerrigan, J. F.Identification of somatic chromosomal abnormalities in hypothalamic hamartoma tissue at the GLI3 locus. Am. J. Hum. Genet. 82: 366-374, 2008.

说明

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

限制

仅限研究用
状态

Lyophilized

溶解方式

Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

浓度

500 μg/mL

缓冲液

Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

储存液

Thimerosal (Merthiolate), Sodium azide

注意事项

This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C,-20 °C

储存方法

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

有效期

12 months
Kumari, Chaurasia, Kumar, Dash: "Anti-apoptotic role of sonic hedgehog on blood platelets." in: Thrombosis research, Vol. 134, Issue 6, pp. 1311-5, (2014) (PubMed).
抗原

GLI3 (GLI Family Zinc Finger 3 (GLI3))

别名

GLI3

背景

Synonyms: Transcriptional activator GLI3,GLI3 form of 190 kDa,GLI3-190,GLI3 full length protein,GLI3FL,Transcriptional repressor GLI3R,GLI3 C-terminally truncated form,GLI3 form of 83 kDa,GLI3-83,GLI3,
Tissue Specificity: Is expressed in a wide variety of normal adult tissues, including lung, colon, spleen, placenta, testis, and myometrium.
Background: GLI3 (Gli-kruppel family member 3), also called ONCOGENE GLI3, encodes a member of the zinc finger gene family related to Kruppel, a gene that is known to regulate development in Drosophila. The GLI3 gene is expressed as an 8.5-kb mRNA in tissues such as testis, myometrium, placenta, and lung, and the protein product (relative molecular mass, 190,000) shows sequence-specific DNA binding. The GLI3 gene is mapped on 7p14.1. GLI3 is homologous to the Drosophila cubitus interruptus (ci) gene product (Ci), which regulates the Patched (pct), gooseberry (gsb), and decapentaplegic (dpp) genes. PKA-dependent processing of vertebrate GLI3 in developing limb generates a potent repressor in a manner antagonized by apparent long-range signaling from posteriorly localized Sonic hedgehog protein. The high relative abundance and potency of GLI3 repressor suggested specialization of GLI3 and its products for negative hedgehog pathway regulation.Coimmunoprecipitation and immunoblot studies showed that GLI3 protein is polyubiquitinated and that its processing depends on proteasome activity. The findings suggested that BTRC is required for GLI3 processing.
Sequence Similarities: Belongs to the GLI C2H2-type zinc-finger protein family.

分子量

33 kDa

UniProt

P10071

途径

Hedgehog Signaling