GLI3 抗体 (N-Term)
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北京 101111
Quick Overview for GLI3 抗体 (N-Term) (ABIN3044156)
抗原
See all GLI3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 40-57, N-Term
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原理
- Anti-Transcriptional activator GLI3 Gli3 Antibody
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序列
- SNEDESPGQT YHRERRNA
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-Transcriptional activator GLI3 Gli3 Antibody (ABIN3044156). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the N-terminus of human Gli3, different from the related rat sequence by two amino acids, and from the related mouse sequence by one amino acid.
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亚型
- IgG
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anti-GLI Family Zinc Finger 3 (GLI3) antibody
GLI3 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-GLI Family Zinc Finger 3 (GLI3) (AA 1-110) antibodyGLI3 适用: 人 WB, IF, ELISA, PLA 宿主: 小鼠 Monoclonal 2C9 unconjugated
anti-GLI Family Zinc Finger 3 (GLI3) (AA 303-561) antibodyGLI3 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-GLI Family Zinc Finger 3 (GLI3) antibodyKD Validated GLI3 适用: 人 WB, FACS 宿主: 兔 Monoclonal 24GB6105 unconjugated Recombinant Antibody
anti-GLI Family Zinc Finger 3 (GLI3) (AA 30-60) antibodyGLI3 适用: 人 WB, IHC, IF, ELISA, FM, MA 宿主: 兔 Polyclonal unconjugated
anti-GLI Family Zinc Finger 3 (GLI3) (AA 1-110) antibodyGLI3 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-GLI Family Zinc Finger 3 (GLI3) (AA 1-110) antibodyGLI3 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1H7 unconjugated
anti-GLI Family Zinc Finger 3 (GLI3) (AA 111-343) antibodyGLI3 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-GLI Family Zinc Finger 3 (GLI3) (AA 800-900) antibodyGLI3 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-GLI Family Zinc Finger 3 (GLI3) (AA 11-60) antibodyGLI3 适用: 人, 小鼠, 大鼠 IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
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说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
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储存液
- Thimerosal (Merthiolate), Sodium azide
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注意事项
- This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. -
有效期
- 12 months
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: "Anti-apoptotic role of sonic hedgehog on blood platelets." in: Thrombosis research, Vol. 134, Issue 6, pp. 1311-5, (2014) (PubMed).
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: "Anti-apoptotic role of sonic hedgehog on blood platelets." in: Thrombosis research, Vol. 134, Issue 6, pp. 1311-5, (2014) (PubMed).
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- GLI3 (GLI Family Zinc Finger 3 (GLI3))
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别名
- GLI3
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背景
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Synonyms: Transcriptional activator GLI3,GLI3 form of 190 kDa,GLI3-190,GLI3 full length protein,GLI3FL,Transcriptional repressor GLI3R,GLI3 C-terminally truncated form,GLI3 form of 83 kDa,GLI3-83,GLI3,
Tissue Specificity: Is expressed in a wide variety of normal adult tissues, including lung, colon, spleen, placenta, testis, and myometrium.
Background: GLI3 (Gli-kruppel family member 3), also called ONCOGENE GLI3, encodes a member of the zinc finger gene family related to Kruppel, a gene that is known to regulate development in Drosophila. The GLI3 gene is expressed as an 8.5-kb mRNA in tissues such as testis, myometrium, placenta, and lung, and the protein product (relative molecular mass, 190,000) shows sequence-specific DNA binding. The GLI3 gene is mapped on 7p14.1. GLI3 is homologous to the Drosophila cubitus interruptus (ci) gene product (Ci), which regulates the Patched (pct), gooseberry (gsb), and decapentaplegic (dpp) genes. PKA-dependent processing of vertebrate GLI3 in developing limb generates a potent repressor in a manner antagonized by apparent long-range signaling from posteriorly localized Sonic hedgehog protein. The high relative abundance and potency of GLI3 repressor suggested specialization of GLI3 and its products for negative hedgehog pathway regulation.Coimmunoprecipitation and immunoblot studies showed that GLI3 protein is polyubiquitinated and that its processing depends on proteasome activity. The findings suggested that BTRC is required for GLI3 processing.
Sequence Similarities: Belongs to the GLI C2H2-type zinc-finger protein family.
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分子量
- 33 kDa
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UniProt
- P10071
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途径
- Hedgehog Signaling
抗原
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