SMAD2 抗体 (N-Term)
(2 references)-
- 抗原 See all SMAD2 抗体
- SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))
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抗原表位
- AA 94-112, N-Term
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适用
- 人, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This SMAD2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
- 原理
- Rabbit IgG polyclonal antibody for Mothers against decapentaplegic homolog 2(SMAD2) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
- 序列
- DQWDTTGLYS FSEQTRSLD
- 交叉反应 （详细）
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Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities. - 产品特性
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Rabbit IgG polyclonal antibody for Mothers against decapentaplegic homolog 2(SMAD2) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: SMAD family member 2
Protein Name: Mothers against decapentaplegic homolog 2 - 纯化方法
- Immunogen affinity purified.
- 免疫原
- A synthetic peptide corresponding to a sequence at the N-terminus of human Smad2(94-112aa DQWDTTGLYSFSEQTRSLD), identical to the related rat and mouse sequences.
- 亚型
- IgG
- Top Product
- Discover our top product SMAD2 Primary Antibody
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anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (AA 181-280) antibody
SMAD2 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 3G9 unconjugated
anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) antibodySMAD2 适用: 人 WB, ELISA, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 5G7 unconjugated
anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (AA 2-467) antibodySMAD2 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (pSer423), (pSer425), (pSer465), (pSer467) antibodySMAD2 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) antibodyKO Validated SMAD2 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (C-Term) antibodySMAD2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (AA 181-280) antibodySMAD2 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 4D10 unconjugated
anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (pSer467) antibodySMAD2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (pThr183), (pThr221) antibodySMAD2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-SMAD, Mothers Against DPP Homolog 2 (SMAD2) (N-Term) antibodySMAD2 适用: 人, 小鼠, 大鼠 WB, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, The detection limit for SMAD2 is approximately 2.5 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. - 说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
- 储存液
- Thimerosal (Merthiolate), Sodium azide
- 注意事项
- This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing. - 有效期
- 12 months
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: "Effect of Kuijie Granule on the Expression of TGF-β/Smads Signaling Pathway in Patients with Ulcerative Colitis." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2016, pp. 2601830, (2016) (PubMed).
: "Tranilast prevents the progression of chronic cyclosporine nephrotoxicity through regulation of transforming growth factor ?/Smad pathways." in: Transplantation proceedings, Vol. 43, Issue 5, pp. 1985-8, (2011) (PubMed).
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: "Effect of Kuijie Granule on the Expression of TGF-β/Smads Signaling Pathway in Patients with Ulcerative Colitis." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2016, pp. 2601830, (2016) (PubMed).
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- 抗原
- SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))
- 别名
- SMAD2 (SMAD2 产品)
- 别名
- JV18 antibody, JV18-1 antibody, MADH2 antibody, MADR2 antibody, hMAD-2 antibody, hSMAD2 antibody, jv18 antibody, Xmad2 antibody, madh2 antibody, madr2 antibody, XSmad2 antibody, hmad-2 antibody, hsmad2 antibody, jv18-1 antibody, smad-2 antibody, fj43c06 antibody, wu:fj43c06 antibody, 7120426M23Rik antibody, Madh2 antibody, Madr2 antibody, Smad-2 antibody, mMad2 antibody, MAD homolog 2 antibody, Mothers against DPP homolog 2 antibody, SMAD 2 antibody, Smad2 antibody, SMAD family member 2 antibody, SMAD family member 2-Z antibody, SMAD family member 2 S homeolog antibody, SMAD2 antibody, SMAD2Z antibody, smad2 antibody, smad2.S antibody, Smad2 antibody
- 背景
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Smad2(Mothers against decapentaplegic homolog 2), also known as MADR2, MADH2, SMAD family member 2 or SMAD2, is a protein that in humans is encoded by the SMAD2 gene. MAD homolog 2 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic'(Mad) and the C. elegans gene Sma. Eppert et al.(1996) mapped the MADR2 gene close to DPC4 at 18q21, a region which is frequently deleted in colorectal cancers. Riggins et al.(1996) mapped the human MADH2 gene to 18q21. Nakao et al.(1997) refined the localization of the SMAD2 gene to 18q21.1, approximately 3 Mb proximal to DPC4, by fluorescence in situ hybridization. SMAD2 mediates the signal of the transforming growth factor(TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation(SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors.
Synonyms: hMAD 2 antibody|hMAD-2 antibody|hSMAD2 antibody|JV18 1 antibody|JV18 antibody|JV18-1 antibody|JV181 antibody|MAD antibody|MAD homolog 2 antibody|MAD Related Protein 2 antibody|Mad-related protein 2 antibody|MADH2 antibody|MADR2 antibody|MGC22139 antibody|MGC34440 antibody|Mothers Against Decapentaplegic Homolog 2 antibody|mothers against DPP homolog 2 antibody|OTTHUMP00000163489 antibody|Sma and Mad related protein 2 antibody|SMAD 2 antibody|SMAD antibody|SMAD family member 2 antibody|SMAD, mothers against DPP homolog 2 antibody|SMAD2 antibody|SMAD2_HUMAN antibody - UniProt
- Q15796
- 途径
- Cell Division Cycle, Hormone Transport, Chromatin Binding, Protein targeting to Nucleus
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