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Beclin 1 抗体 (Middle Region)

Name: Beclin 1 抗体 (Middle Region)
SKU: ABIN3044080
Availability: OutOfStock

This anti-Beclin 1 antibody is a 兔多克隆 antibody detecting Beclin 1 in WB. Suitable for 人, 小鼠和大鼠. This Primary Antibody has been cited in 5+ publications.

Anti-Beclin 1 antibody, Western blotting Lane 1: HELA Cell Lysate Lane 2: SW620 Cell Lysate Lane 3: PANC Cell Lysate (Beclin 1 抗体 (Middle Region))

2 images

(5 references)

产品编号 ABIN3044080

发货至: 中国

Datasheet as PDF

Quick Overview for Beclin 1 抗体 (Middle Region) (ABIN3044080)

抗原

See all Beclin 1 (BECN1) 抗体

Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))

适用

人, 小鼠, 大鼠

宿主

兔

克隆类型

多克隆

标记

This Beclin 1 antibody is un-conjugated

应用范围

Western Blotting (WB)

Discover our top product BECN1 Primary Antibody

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AA 222-237, Middle Region

原理

Anti-Beclin 1/BECN1 Antibody Picoband®

序列

LDQEEAQYQR EYSEFK

交叉反应（详细）

No cross-reactivity with other proteins

产品特性

Anti-Beclin 1/BECN1 Antibody (ABIN3044080). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

纯化方法

Immunogen affinity purified.

免疫原

A synthetic peptide corresponding to a sequence in the middle region of human Beclin 1, identical to the related rat and mouse sequences.

亚型

IgG
anti-Beclin 1, Autophagy Related (BECN1) (AA 201-330) antibody
BECN1 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, ICC, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Beclin 1, Autophagy Related (BECN1) antibody
BECN1 适用: 人, 大鼠, 犬 WB, IHC, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal 1F1 unconjugated

anti-Beclin 1, Autophagy Related (BECN1) antibody
BECN1 适用: 人, 大鼠, 猴 WB, IHC, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal 3F3 unconjugated

anti-Beclin 1, Autophagy Related (BECN1) antibody
BECN1 适用: 人, 大鼠, 猴, 犬 WB, IHC, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal 3C3 unconjugated

anti-Beclin 1, Autophagy Related (BECN1) antibody
BECN1 适用: 人 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 2A4 unconjugated

anti-Beclin 1, Autophagy Related (BECN1) (AA 1-354) antibody
BECN1 适用: 人, 小鼠, 大鼠 WB, IF, FACS, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-Beclin 1, Autophagy Related (BECN1) (AA 210-239) antibody
BECN1 适用: 人, 小鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB7463 unconjugated

anti-Beclin 1, Autophagy Related (BECN1) (AA 210-239) antibody
BECN1 适用: 人, 小鼠 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated

anti-Beclin 1, Autophagy Related (BECN1) (AA 1-273) antibody
BECN1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Beclin 1, Autophagy Related (BECN1) (C-Term) antibody
BECN1 适用: 人, 小鼠 WB, ELISA, IHC, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated

应用备注

Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Liang, X. H., Kleeman, L. K., Jiang, H. H., Gordon, G., Goldman, J. E., Berry, G., Herman, B., Levine, B.Protection against fatal Sindbis virus encephalitis by beclin, a novel Bcl-2-interacting protein. J. Virol. 72: 8586-8596, 1998.

说明

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

限制

仅限研究用
状态

Lyophilized

溶解方式

Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

浓度

500 μg/mL

缓冲液

Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

储存液

Thimerosal (Merthiolate), Sodium azide

注意事项

This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C,-20 °C

储存方法

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

有效期

12 months
Qi, Xue, Lv, Sun, Du, Cai, Li, Gu, Wang: "Ginkgolic acids induce HepG2 cell death via a combination of apoptosis, autophagy and the mitochondrial pathway." in: Oncology letters, Vol. 15, Issue 5, pp. 6400-6408, (2018) (PubMed).

Shen, Cai, Liu, Li, Gan, Li, Wang, Guo, Zhou, Lu, Sun, Li: "Ube2v1-mediated ubiquitination and degradation of Sirt1 promotes metastasis of colorectal cancer by epigenetically suppressing autophagy." in: Journal of hematology & oncology, Vol. 11, Issue 1, pp. 95, (2018) (PubMed).

Wang, Ma, Hu, Xie, Wu, Zeng, Song: "Bifidobacterial recombinant thymidine kinase-ganciclovir gene therapy system induces FasL and TNFR2 mediated antitumor apoptosis in solid tumors." in: BMC cancer, Vol. 16, pp. 545, (2017) (PubMed).

Sun, Gao, Meng, Xu, Wang, Gu, Guo, Shao, Yan, Jiang, Zheng: "Antagomirs Targeting MiroRNA-134 Attenuates Epilepsy in Rats through Regulation of Oxidative Stress, Mitochondrial Functions and Autophagy." in: Frontiers in pharmacology, Vol. 8, pp. 524, (2017) (PubMed).

Bu, Zhao, Zhang, Wang, Li, Yan: "Recombinant Newcastle disease virus (rL-RVG) triggers autophagy and apoptosis in gastric carcinoma cells by inducing ER stress." in: American journal of cancer research, Vol. 6, Issue 5, pp. 924-36, (2016) (PubMed).
抗原

Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))

别名

BECN1

背景

Synonyms: Beclin-1;Coiled-coil myosin-like BCL2-interacting protein;Protein GT197;BECN1;GT197;
Tissue Specificity: Ubiquitous.
Background: Bellini-1 (beclin 1, autophagy related) is a protein that in humans is encoded by the BECN1 gene, also known as BECN1, ATG6, VPS30, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197, autophagy-related gene (Atg) 6. Beclin-1 and its binding partner class III phosphoinositide 3-kinase (PI3K), also named Vps34, are required for the initiation of the formation of the autophagosome in autophagy. Human beclin encodes a novel 450-amino acid protein containing a coiled-coil region, within which it has limited homology to myosin-like proteins. Northern blot analysis of mouse and adult human tissues revealed widespread beclin expression. A 2.2-kb transcript was present at highest levels in human skeletal muscle and at detectable levels in all tissues examined. In some tissues, additional 1.7- and 1.4-kb transcripts were observed, suggesting the presence of alternatively spliced transcripts. Immunoperoxidase staining of human hippocampus and frontal cortex sections revealed beclin immunoreactivity in many neurons throughout these regions, as well as in some glial cells. Beclin protects against infection by a neurovirulent strain of Sindbis virus that is known to overcome the protective effects of Bcl2. Liang et al. showed that beclin reduced Sindbis virus replication in mouse brain and reduced Sindbis virus-induced cell death in mouse brain.

分子量

46 kDa

UniProt

Q14457

途径

Autophagy