MAP3K3 抗体 (N-Term)
Quick Overview for MAP3K3 抗体 (N-Term) (ABIN3044008)
抗原
See all MAP3K3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 11-25, N-Term
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原理
- Anti-MEKK3/MAP3K3 Antibody Picoband®
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序列
- MKDLVALQMS RRTRL
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-MEKK3/MAP3K3 Antibody (ABIN3044008). Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the N-terminus of mouse MEKK3, different from the related rat sequence by two amino acids.
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亚型
- IgG
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anti-Mitogen-Activated Protein Kinase Kinase Kinase 3 (MAP3K3) (AA 1-220) antibody
MAP3K3 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase 3 (MAP3K3) antibodyMAP3K3 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase 3 (MAP3K3) (AA 1-90) antibodyMAP3K3 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase 3 (MAP3K3) (N-Term) antibodyMAP3K3 适用: 人, 小鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase 3 (MAP3K3) (Center) antibodyMAP3K3 适用: 人, 小鼠, 大鼠 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase 3 (MAP3K3) (Internal Region) antibodyMAP3K3 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase 3 (MAP3K3) antibodyMAP3K3 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase 3 (MAP3K3) (Internal Region) antibodyMAP3K3 适用: 人, 小鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase 3 (MAP3K3) (AA 1-89) antibodyMAP3K3 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1H3 unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase 3 (MAP3K3) (AA 1-90) antibodyMAP3K3 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
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应用备注
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Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Rat, Mouse
Western blot, 0.1-0.5 μg/mL, Mouse, Rat
1. Channavajhala, P. L., Rao, V. R., Spaulding, V., Lin, L. L., Zhang, Y. G. hKSR-2 inhibits MEKK3-activated MAP kinase and NF-kappa-B pathways in inflammation. Biochem. Biophys. Res. Commun. 334: 1214-1218, 2005. 2. Puffenberger, E. G., Strauss, K. A., Ramsey, K. E., Craig, D. W., Stephan, D. A., Robinson, D. L., Hendrickson, C. L., Gottlieb, S., Ramsay, D. A., Siu, V. M., Heuer, G. G., Crino, P. B., Morton, D. H. Polyhydramnios, megalencephaly and symptomatic epilepsy caused by a homozygous 7-kilobase deletion in LYK5. Brain 130: 1929-1941, 2007. 3. Uhlik, M. T., Abell, A. N., Johnson, N. L., Sun, W., Cuevas, B. D., Lobel-Rice, K. E., Horne, E. A., Dell'Acqua, M. L., Johnson, G. L. Rac-MEKK3-MKK3 scaffolding for p38 MAPK activation during hyperosmotic shock. Nature Cell Biol. 5: 1104-1110, 2003. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
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储存液
- Thimerosal (Merthiolate), Sodium azide
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注意事项
- This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. -
有效期
- 12 months
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- MAP3K3 (Mitogen-Activated Protein Kinase Kinase Kinase 3 (MAP3K3))
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别名
- MAP3K3
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背景
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Synonyms: Mitogen-activated protein kinase kinase kinase 3,2.7.11.25,MAPK/ERK kinase kinase 3,MEK kinase 3,MEKK 3,Map3k3,Mekk3,
Background: MAP3K3 (Mitogen-activated protein kinase kinase kinase 3), also known as MAP/ERK KINASE KINASE 3, MEKK3 or MAPKKK3, is an enzyme that in humans is encoded by the MAP3K3 gene. MAP3K3 is contiguously distal to LYK5 on chromosome 17 (Puffenberger et al., 2007). By yeast 2-hybrid analysis of a mouse T-cell cDNA library, Uhlik et al. (2003) showed that a C-terminal fragment of mouse Osm (CCM2) interacted with Mekk3, which activates p38 in response to sorbitol-induced hyperosmotic conditions. Mekk3 and Osm colocalized in the cytoplasmic compartment of cotransfected cells, and the Mekk3-Osm complex was recruited to Rac1 and cytoskeletal actin-containing membrane ruffles in response to sorbitol treatment. Protein interaction assays showed that Osm interacted ly with the Mekk3 substrate Mkk3 (MAP2K3), with actin, and with both GDP- and GTP-loaded Rac1. Uhlik et al. (2003) concluded that the RAC1-OSM-MEKK3-MKK3 complex is required for regulation of p38 activity in response to osmotic shock.
Sequence Similarities: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
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分子量
- 71 kDa
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UniProt
- Q61084
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途径
- MAPK Pathway
抗原
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