YBX1 抗体 (C-Term)
Quick Overview for YBX1 抗体 (C-Term) (ABIN3043966)
抗原
See all YBX1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 293-320, C-Term
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原理
- Anti-YB1/YBX1 Antibody Picoband®
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序列
- ENPKPQDGKE TKAADPPAEN SSAPEAEQ
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-YB1/YBX1 Antibody Picoband® (ABIN3043966). Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human YB1, identical to the related mouse and rat sequences.
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亚型
- IgG
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anti-Y Box Binding Protein 1 (YBX1) (AA 100-200) antibody
YBX1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Y Box Binding Protein 1 (YBX1) antibodyYBX1 适用: 人 WB, IHC, IF, IP, ChIP 宿主: 兔 Monoclonal unconjugated
anti-Y Box Binding Protein 1 (YBX1) (AA 100-200) antibodyYBX1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Y Box Binding Protein 1 (YBX1) (AA 51-139) antibodyYBX1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 4C7 unconjugated
anti-Y Box Binding Protein 1 (YBX1) antibodyYBX1 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal YBX1-2430 unconjugated
anti-Y Box Binding Protein 1 (YBX1) antibodyYBX1 适用: 人, 大鼠, 小鼠, Cow, 犬, 小鸡, Pig WB, IHC (p), FACS 宿主: 兔 Polyclonal unconjugated
anti-Y Box Binding Protein 1 (YBX1) (pSer102) antibodyYBX1 适用: 人, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Y Box Binding Protein 1 (YBX1) antibodyYBX1 适用: 人, 大鼠, 小鼠 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Y Box Binding Protein 1 (YBX1) antibodyYBX1 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal rYBX1-2430 unconjugated Recombinant Antibody
anti-Y Box Binding Protein 1 (YBX1) (AA 276-305), (C-Term) antibodyYBX1 适用: 人 WB, IF 宿主: 兔 Polyclonal RB14114 unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human, -
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
1. Bhullar, J., Sollars, V. E. YBX1 expression and function in early hematopoiesis and leukemic cells. Immunogenetics 63: 337-350, 2011. 2. Fukada, T., Tonks, N. K. Identification of YB-1 as a regulator of PTP1B expression: implications for regulation of insulin and cytokine signaling. EMBO J. 22: 479-493, 2003. 3. Makino, Y., Ohga, T., Toh, S., Koike, K., Okumura, K., Wada, M., Kuwano, M., Kohno, K. Structural and functional analysis of the human Y-box binding protein (YB-1) gene promoter. Nucleic Acid Res. 24: 1873-1878, 1996. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- YBX1 (Y Box Binding Protein 1 (YBX1))
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别名
- YBX1
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背景
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Synonyms: Nuclease-sensitive element-binding protein 1,CCAAT-binding transcription factor I subunit A,CBF-A,DNA-binding protein B,DBPB,Enhancer factor I subunit A,EFI-A,Y-box transcription factor,Y-box-binding protein 1,YB-1,YBX1,NSEP1, YB1,
Tissue Specificity: Expressed in plasma cells in rheumatoid synovium (PubMed:11460154). Over-expressed in primary breast cancer and metastatic breast cancer cells (PubMed:25280941). Isoform 1 and isoform 2 are expressed at higher level in proliferating as compared to confluent quiescent endothelial cells (PubMed:19416856). .
Background: YBX1 (Y box binding protein 1), commonly referred to as "YB-1" by researchers, is a human protein. YB1 binding has an absolute requirement for the CCAAT box and relative specificity for the Y box. It has a molecular mass of 35,414 and contains 18 % basic residues and putative nuclear localization signals. The YBX1 gene is mapped on 1p34.2. Ybx1 was highly expressed in mouse erythroid myeloid lymphoid clone-1 (EML), a hematopoietic precursor cell line, but that it was downregulated in myeloid progenitors and in Gmcsf-treated EML cells during myeloid differentiation. Ybx1 was expressed at high levels in myeloid leukemic cells at different developmental stages. Knockdown of YBX1 in a human leukemic cell line inhibited proliferation ability, induced apoptosis, and induced megakaryocytic differentiation in response to arsenic trioxide treatment. YBX1 is downregulated during myeloid differentiation and aberrant YBX1 expression in leukemic cells may contribute to leukemia development by blocking differentiation.
Sequence Similarities: Contains 1 CSD (cold-shock) domain.
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分子量
- 50 kDa
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基因ID
- 4904
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UniProt
- P67809
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途径
- Regulation of Muscle Cell Differentiation
抗原
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