TFAM 抗体 (N-Term)
(2 references)Quick Overview for TFAM 抗体 (N-Term) (ABIN3043945)
抗原
See all TFAM 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 214-241, N-Term
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原理
- Anti-mtTFA/TFAM Antibody Picoband®
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序列
- EMKSWEEQMI EVGRKDLLRR TIKKQRKY
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-mtTFA/TFAM Antibody Picoband® (ABIN3043945). Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the N-terminus of human mtTFA, different from the related mouse and rat sequences by five amino acids.
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亚型
- IgG
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anti-Transcription Factor A, Mitochondrial (TFAM) (N-Term) antibody
TFAM 适用: 人, 小鼠, 兔, Cow, 犬, 豚鼠, 马, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Transcription Factor A, Mitochondrial (TFAM) (AA 43-243) antibodyTFAM 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Transcription Factor A, Mitochondrial (TFAM) (AA 1-220) antibodyTFAM 适用: 人 WB, IHC, IF, ChIP 宿主: 兔 Polyclonal unconjugated
anti-Transcription Factor A, Mitochondrial (TFAM) (AA 1-246) antibodyTFAM 适用: 人 WB, IF, IHC (p) 宿主: 小鼠 Polyclonal unconjugated
anti-Transcription Factor A, Mitochondrial (TFAM) antibodyKO Validated TFAM 适用: 人 WB, IF, IP, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Transcription Factor A, Mitochondrial (TFAM) (AA 227-246), (C-Term) antibodyTFAM 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Transcription Factor A, Mitochondrial (TFAM) (AA 216-246), (C-Term) antibodyTFAM 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB27631 unconjugated
anti-Transcription Factor A, Mitochondrial (TFAM) (AA 1-246) antibodyTFAM 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Transcription Factor A, Mitochondrial (TFAM) antibodyTFAM 适用: 人 WB, IHC, IF, ChIP 宿主: 兔 Monoclonal unconjugated
anti-Transcription Factor A, Mitochondrial (TFAM) (AA 216-246) antibodyTFAM 适用: 人 WB, IHC, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
1. Larsson, N.-G., Oldfors, A., Garman, J. D., Barsh, G. S., Clayton, D. A. Down-regulation of mitochondrial transcription factor A during spermatogenesis in humans. Hum. Molec. Genet. 6: 185-191, 1997. 2. Milatovich, A., Parisi, M. A., Poulton, J., Clayton, D. A., Francke, U. Sequences homologous to MTTF1, mitochondrial transcription factor 1, are located on human chromosomes 7 (7pter-cen), 10 and 11 (11cen-qter). (Abstract) Cytogenet. Cell Genet. 58: 1924 only, 1992. 3. Scott, A. F. Personal Communication. Baltimore, Md. 9/20/2007. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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: "Roles of mitochondrial transcription factor A and microRNA‑590‑3p in the development of colon cancer." in: Molecular medicine reports, Vol. 14, Issue 6, pp. 5475-5480, (2017) (PubMed).
: "Roles of mitochondrial transcription factor A and microRNA-590-3p in the development of bladder cancer." in: Oncology letters, Vol. 6, Issue 2, pp. 617-623, (2013) (PubMed).
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: "Roles of mitochondrial transcription factor A and microRNA‑590‑3p in the development of colon cancer." in: Molecular medicine reports, Vol. 14, Issue 6, pp. 5475-5480, (2017) (PubMed).
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- TFAM (Transcription Factor A, Mitochondrial (TFAM))
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别名
- TFAM
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背景
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Synonyms: Transcription factor A, mitochondrial,mtTFA,Mitochondrial transcription factor 1,MtTF1,Transcription factor 6,TCF-6,Transcription factor 6-like 2,TFAM,TCF6, TCF6L2,
Tissue Specificity: Ubiquitously expressed. .
Background: TFAM (Transcription factor A, mitochondrial), also known as TCF6 or TCF6L2, is a 162-amino acid protein that activates transcription of each mitochondrial DNA (mtDNA) strand by binding to an element of approximately 30 nucleotides present in both the light-strand and the heavy-strand promoters. By Southern blot analysis of restriction enzyme digests of human/Chinese hamster somatic cell hybrid lines, Milatovich et al. (1992) mapped TFAM sequences, which they called MTTF1, to 3 different chromosomes: chromosomes 10, 7p, and 11q. By PCR-based screening of a somatic cell hybrid panel and by fluorescence in situ hybridization, Scott (2007) stated that the sequences mapped to chromosomes 7p (TCF6L1) and 11q (MTTF1, or TCF6L3) are pseudogenes. Larsson et al. (1997) mapped the mouse mitochondrial transcription factor A gene (Tfam) to the central part of mouse chromosome 10. This region exhibits syntenic homology with human 10q21. Mitochondrial transcription factor A is a key activator of mitochondrial transcription in mammals. It also has a role in mitochondrial DNA replication, since transcription generates an RNA primer necessary for initiation of mtDNA replication.
Sequence Similarities: Belongs to the G-protein coupled receptor 1 family.
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分子量
- 24 kDa
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基因ID
- 7019
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UniProt
- Q00059
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途径
- Chromatin Binding
抗原
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