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TFAM 抗体 (N-Term)

Name: TFAM 抗体 (N-Term)
SKU: ABIN2777277
Availability: OutOfStock

TFAM 适用: 人, 小鼠, 兔, Cow, 犬, 豚鼠, Pig, 马 WB 宿主: 兔 Polyclonal unconjugated

Western Blotting (WB) image for anti-Transcription Factor A, Mitochondrial (TFAM) (N-Term) antibody (ABIN2777277) (TFAM 抗体 (N-Term))

TFAM antibody - N-terminal region validated by WB using bEND3 cell lysate at lane1:10 ug, lane2:20 ug. (TFAM 抗体 (N-Term))

Lanes: Lane 1 and 2: HepG2 cell lysate Lane 3 and 4: Hep3B cell lysate Lane 5 and 6: K562 cell lysate Primary Antibody Dilution: 1:1000 Secondary Antibody: Secondary Antibody Dilution: 1:1000 Gene Name: TFAM Submitted by: Anonymous (TFAM 抗体 (N-Term))

WB Suggested Anti-TFAM Antibody Titration: 0. (TFAM 抗体 (N-Term))

6 images

(10 references)

产品编号 ABIN2777277

发货至: 中国

Datasheet as PDF

抗原 See all TFAM 抗体

TFAM (Transcription Factor A, Mitochondrial (TFAM))
抗原表位
15

13

9

7

6

4

4

4

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
N-Term

适用

人, 小鼠, 兔, Cow, 犬, 豚鼠, Pig, 马
宿主
89

4
兔
Compatible Secondaries
克隆类型
79

14
多克隆
标记
42

11

9

4

3

3

3

3

3

3

2

2

1

1

1

1

1
This TFAM antibody is un-conjugated
应用范围
73

39

28

28

19

16

15

14

14

8

5

4

3

2

2

1
Western Blotting (WB)

序列

AKTTELIRRI AQRWRELPDS KKKIYQDAYR AEWQVYKEEI SRFKEQLTPS

预测反应

Cow: 92%, Dog: 83%, Guinea Pig: 75%, Horse: 92%, Human: 100%, Mouse: 75%, Pig: 92%, Rabbit: 83%

产品特性

This is a rabbit polyclonal antibody against Transcription Factor AntibodiesAM. It was validated on Western Blot using a cell lysate as a positive control.

纯化方法

Affinity Purified

免疫原

The immunogen is a synthetic peptide directed towards the N terminal region of human TFAM
anti-Transcription Factor A, Mitochondrial (TFAM) (AA 43-243) antibody
TFAM 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Transcription Factor A, Mitochondrial (TFAM) (AA 1-220) antibody
TFAM 适用: 人 WB, IHC, IF, ChIP 宿主: 兔 Polyclonal unconjugated

anti-Transcription Factor A, Mitochondrial (TFAM) (AA 1-246) antibody
TFAM 适用: 人 WB, IF, IHC (p) 宿主: 小鼠 Polyclonal unconjugated

anti-Transcription Factor A, Mitochondrial (TFAM) antibody
KO Validated TFAM 适用: 人 WB, IF, IP, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Transcription Factor A, Mitochondrial (TFAM) (AA 227-246), (C-Term) antibody
TFAM 适用: 人 WB, ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Transcription Factor A, Mitochondrial (TFAM) (AA 216-246), (C-Term) antibody
TFAM 适用: 人 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB27631 unconjugated

anti-Transcription Factor A, Mitochondrial (TFAM) (AA 1-246) antibody
TFAM 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated

anti-Transcription Factor A, Mitochondrial (TFAM) antibody
TFAM 适用: 人 WB, IHC, IF, ChIP 宿主: 兔 Monoclonal unconjugated

anti-Transcription Factor A, Mitochondrial (TFAM) (AA 216-246) antibody
TFAM 适用: 人 WB, IHC, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated

anti-Transcription Factor A, Mitochondrial (TFAM) (AA 214-241), (N-Term) antibody
TFAM 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

应用备注

Optimal working dilutions should be determined experimentally by the investigator.

说明

Antigen size: 246 AA

限制

仅限研究用
状态

Liquid

浓度

Lot specific

缓冲液

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freeze-thaw cycles.

储存条件

-20 °C

储存方法

For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Ogasawara, Liu, Pelicano, Hammoudi, Croce, Keating, Huang: "Alterations of mitochondrial biogenesis in chronic lymphocytic leukemia cells with loss of p53." in: Mitochondrion, Vol. 31, pp. 33-39, (2016) (PubMed).

Kemper, Stirone, Krause, Duckles, Procaccio: "Genomic and non-genomic regulation of PGC1 isoforms by estrogen to increase cerebral vascular mitochondrial biogenesis and reactive oxygen species protection." in: European journal of pharmacology, Vol. 723, pp. 322-9, (2014) (PubMed).

Vettor, Valerio, Ragni, Trevellin, Granzotto, Olivieri, Tedesco, Ruocco, Fossati, Fabris, Serra, Carruba, Nisoli: "Exercise training boosts eNOS-dependent mitochondrial biogenesis in mouse heart: role in adaptation of glucose metabolism." in: American journal of physiology. Endocrinology and metabolism, Vol. 306, Issue 5, pp. E519-28, (2014) (PubMed).

Kemper, Zhao, Duckles, Krause: "Endogenous ovarian hormones affect mitochondrial efficiency in cerebral endothelium via distinct regulation of PGC-1 isoforms." in: Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, Vol. 33, Issue 1, pp. 122-8, (2013) (PubMed).

Teodoro, Duarte, Gomes, Varela, Peixoto, Rolo, Palmeira: "Berberine reverts hepatic mitochondrial dysfunction in high-fat fed rats: a possible role for SirT3 activation." in: Mitochondrion, Vol. 13, Issue 6, pp. 637-46, (2013) (PubMed).

Miyazaki, Iwasawa, Nakashima, Mori, Shigemoto, Nakamura, Katagiri, Takayanagi, Tanaka: "Intracellular and extracellular ATP coordinately regulate the inverse correlation between osteoclast survival and bone resorption." in: The Journal of biological chemistry, Vol. 287, Issue 45, pp. 37808-23, (2012) (PubMed).

Lu, Chen, Zhang, Wang, Luo, Huang: "ZNF143 transcription factor mediates cell survival through upregulation of the GPX1 activity in the mitochondrial respiratory dysfunction." in: Cell death & disease, Vol. 3, pp. e422, (2012) (PubMed).

Walters, Wallace: "Urea cycle gene expression is suppressed by PFOA treatment in rats." in: Toxicology letters, Vol. 197, Issue 1, pp. 46-50, (2010) (PubMed).

Walters, Bjork, Wallace: "Perfluorooctanoic acid stimulated mitochondrial biogenesis and gene transcription in rats." in: Toxicology, Vol. 264, Issue 1-2, pp. 10-5, (2009) (PubMed).

Shi, Burkart, Nicoloro, Czech, Straubhaar, Corvera: "Paradoxical effect of mitochondrial respiratory chain impairment on insulin signaling and glucose transport in adipose cells." in: The Journal of biological chemistry, Vol. 283, Issue 45, pp. 30658-67, (2008) (PubMed).
抗原

TFAM (Transcription Factor A, Mitochondrial (TFAM))

别名

TFAM (TFAM 产品)

别名

tcf6 antibody, mttf1 antibody, mttfa antibody, tcf6l1 antibody, tcf6l2 antibody, tcf6l3 antibody, mttfa-A antibody, xl-mtTFA antibody, MGC153358 antibody, zgc:153358 antibody, MTTF1 antibody, MTTFA antibody, TCF6 antibody, TCF6L1 antibody, TCF6L2 antibody, TCF6L3 antibody, AI661103 antibody, Hmgts antibody, mtTFA antibody, tsHMG antibody, Mttfa antibody, transcription factor A, mitochondrial antibody, transcription factor A, mitochondrial L homeolog antibody, TFAM antibody, tfam.L antibody, tfam antibody, Tfam antibody

背景

The function remains unknown.This gene encodes a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. Studies in mice have demonstrated that this gene product is required to regulate the mitochondrial genome copy number and is essential for embryonic development. A mouse model for Kearns-Sayre syndrome was produced when expression of this gene was eliminated by targeted disruption in heart and muscle cells.
Alias Symbols: TCF6, MtTF1, mtTFA, TCF6L1, TCF6L2, TCF6L3
Protein Interaction Partner: AGTRAP, FBXW11, ARL6IP1, NEDD1, PPP2R1A, GRSF1, UBC, MDM2, SUZ12, EED, PAN2, MYC, FN1, C1QBP, CAND1, COPS5, CUL3, ELAVL1, ICT1, TFB2M, TFB1M, PNP, NRF1,
Protein Size: 246

分子量

29 kDa

基因ID

7019

NCBI登录号

NM_003201, NP_003192

UniProt

Q00059

途径

Chromatin Binding