Parkin 抗体 (AA 23-416)
Quick Overview for Parkin 抗体 (AA 23-416) (ABIN3043896)
抗原
See all Parkin (PARK2) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 23-416
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原理
- Anti-Parkin/PRKN Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-Parkin/PRKN Antibody Picoband® (ABIN3043896). Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human Parkin recombinant protein (Position: I23-K416). Human Parkin shares 82% and 84% amino acid (aa) sequence identity with mouse and rat Parkin, respectively.
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亚型
- IgG
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anti-Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2) (AA 288-387) antibody
PARK2 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1H4 unconjugated
anti-Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2) (AA 1-300) antibodyPARK2 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2) (AA 387-417), (C-Term) antibodyPARK2 适用: 人, 小鼠 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB07373 unconjugated
anti-Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2) (AA 387-417) antibodyPARK2 适用: 人, 小鼠 WB, ELISA, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2) (AA 111-140) antibodyPARK2 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2) (AA 361-465) antibodyPARK2 适用: 大鼠 WB, ELISA, IF (cc), IF (p), FACS, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2) (AA 1-465) antibodyPARK2 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2) (AA 1-144) antibodyPARK2 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 6F5D2 unconjugated
anti-Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2) (pSer378) antibodyPARK2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2) (AA 311-360) antibodyPARK2 适用: 人, 小鼠, 大鼠 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunocytochemistry , 0.5-1 μg/mL, Mouse, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Huynh, D. P., Scoles, D. R., Ho, T. H., Del Bigio, M. R., Pulst, S.-M. Parkin is associated with actin filaments in neuronal and nonneural cells. Ann. Neurol. 48: 737-744, 2000. 2. Kitada T, Asakawa S, Hattori N, Matsumine H, Yamamura Y, Minoshima S et al. (Apr 1998). "Mutations in the parkin gene cause autosomal recessive juvenile parkinsonism". Nature 392 (6676): 605-608. 3. Rothfuss, O., Fischer, H., Hasegawa, T., Maisel, M., Leitner, P., Miesel, F., Sharma, M., Bornemann, A., Berg, D., Gasser, T., Patenge, N. Parkin promotes mitochondrial genome integrity and supports mitochondrial DNA repair. Hum. Molec. Genet. 18: 3832-3850, 2009. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- Parkin (PARK2) (Parkinson Protein 2, E3 Ubiquitin Protein Ligase (Parkin) (PARK2))
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别名
- PARK2
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背景
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Synonyms: E3 ubiquitin-protein ligase parkin,Parkin,6.3.2.-,Parkinson juvenile disease protein 2,Parkinson disease protein 2,PARK2,PRKN,
Tissue Specificity: Highly expressed in the brain including the substantia nigra. Expressed in heart, testis and skeletal muscle. Expression is down-regulated or absent in tumor biopsies, and absent in the brain of PARK2 patients. Overexpression protects dopamine neurons from kainate-mediated apoptosis. Found in serum (at protein level). .
Background: Parkin is a RING domain-containing E3 ubiquitin ligase involved in proteasome-dependent degradation of proteins. It is mapped to 6q26. This gene is important for mitochondrial quality control by lysosome-dependent degradation of damaged mitochondria through autophagy, or mitophagy. Parkin is expressed in neuronal processes and cell bodies of neurons, but not glial cells, in the midbrain, basal ganglia, cerebral cortex, and cerebellum. Parkin assimilated with actin filaments, suggesting that it is a cytoskeletal-associated protein. Parkin is identified as a transcriptional repressor of p53 independent of its ubiquitin ligase function. It also has been found that parkin was associated physically with mitochondrial DNA (mtDNA) in proliferating as well as in differentiated SH-SY5Y neuroblastoma cells.
Sequence Similarities: Belongs to the RBR family. Parkin subfamily.
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分子量
- 66 kDa
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基因ID
- 5071
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UniProt
- O60260
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途径
- Autophagy, Ubiquitin Proteasome Pathway
抗原
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