HSPD1 抗体 (AA 260-496)
(5 references)Quick Overview for HSPD1 抗体 (AA 260-496) (ABIN3043854)
抗原
See all HSPD1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 260-496
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原理
- Anti-Hsp60/HSPD1 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-Hsp60/HSPD1 Antibody Picoband® (ABIN3043854). Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human Hsp60 recombinant protein (Position: A260-Q496). Human Hsp60 shares 97% amino acid (aa) sequence identity with both mouse and rat Hsp60.
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亚型
- IgG
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anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) (AA 501-573) antibody
HSPD1 适用: 人, 小鼠, 大鼠, Pig WB, ELISA, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) (AA 27-240) antibodyHSPD1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) antibodyHSPD1 适用: 人 WB, IHC, FACS, ELISA, IP 宿主: 小鼠 Monoclonal LK1 unconjugated
anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) antibodyHSPD1 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, FACS, ELISA, ICC 宿主: 小鼠 Monoclonal 3G8 unconjugated
anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) (AA 172-343) antibodyHSPD1 适用: 人 WB, IHC, FACS, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) (AA 1-573) antibodyHSPD1 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) (AA 316-573) antibodyHSPD1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) antibodyHSPD1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, IEM 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) (AA 1-573) antibodyHSPD1 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) antibodyHSPD1 适用: 人, 小鼠, 大鼠, 小鸡, 猴, Hamster, 犬, Cow, 兔, Pig, 绵羊, Drosophila melanogaster, 非洲爪蟾 WB, IHC, IF, FACS, ISt, StM 宿主: 小鼠 Monoclonal LK1 unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
1. Cheng, M. Y., Hartl, F.-U., Martin, J., Pollock, R. A., Kalousek, F., Neupert, W., Hallberg, E. M., Hallberg, R. L., Horwich, A. L. : Mitochondrial heat-shock protein hsp60 is essential for assembly of proteins imported into yeast mitochondria. Nature 337: 620-625, 1989. 2. Zanin-Zhorov, A., Cahalon, L., Tal, G., Margalit, R., Lider, O., Cohen, I. R. : Heat shock protein 60 enhances CD4+CD25+ regulatory T cell function via innate TLR2 signaling. J. Clin. Invest. 116: 2022-2032, 2006. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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: "Molecular cloning, characterization, and expression of hsp60 in caudal fin regeneration of Misgurnus anguillicaudatus." in: Molecular and cellular biochemistry, Vol. 387, Issue 1-2, pp. 143-50, (2014) (PubMed).
: "Hsp60 in caudal fin regeneration from Paramisgurnus dabryanus: molecular cloning and expression characterization." in: Fish & shellfish immunology, Vol. 36, Issue 2, pp. 401-8, (2014) (PubMed).
: "Hepatic mitochondrial and ER stress induced by defective PPARα signaling in the pathogenesis of hepatic steatosis." in: American journal of physiology. Endocrinology and metabolism, Vol. 306, Issue 11, pp. E1264-73, (2014) (PubMed).
: "Lecithin:cholesterol acyltransferase deficiency protects against cholesterol-induced hepatic endoplasmic reticulum stress in mice." in: The Journal of biological chemistry, Vol. 287, Issue 24, pp. 20755-68, (2012) (PubMed).
: "Cloning of the heat shock protein 60 gene from the stem borer, Chilo suppressalis, and analysis of expression characteristics under heat stress." in: Journal of insect science (Online), Vol. 10, pp. 100, (2010) (PubMed).
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: "Molecular cloning, characterization, and expression of hsp60 in caudal fin regeneration of Misgurnus anguillicaudatus." in: Molecular and cellular biochemistry, Vol. 387, Issue 1-2, pp. 143-50, (2014) (PubMed).
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- HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))
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别名
- HSPD1
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背景
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Synonyms: 60 kDa heat shock protein, mitochondrial,60 kDa chaperonin,Chaperonin 60,CPN60,Heat shock protein 60,HSP-60,Hsp60,HuCHA60,Mitochondrial matrix protein P1,P60 lymphocyte protein,HSPD1,HSP60,
Tissue Specificity: Ubiquitously expressed in normal tissues but is overexpressed in neurons affected in AD. .
Background: HSP60 is a member of the chaperonin class of protein factors, which include the Escherichia coli groEL protein and the Rubisco subunit-binding protein of chloroplasts. It acts as a costimulator of human regulatory CD4-positive/CD25 -positive T cells, which inhibit lymphoproliferation and IFNG and TNF secretion by CD4-positive and CD8-positive T cells. HSP60 enhances Treg activity via TLR2, leading to activation of an intracellular signaling cascade that included p38, as well as inhibition of ERK phosphorylation. Suppression of target T cells is mediated by both cell-to-cell contact and by secretion of TGFB and IL10, and it leads to downregulation of ERK, NFKB, and TBET expression. The self-molecule HSP60 can downregulate adaptive immune responses by upregulating Tregs through TLR2 signaling.
Sequence Similarities: Belongs to the chaperonin (HSP60) family.
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分子量
- 61 kDa
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基因ID
- 3329
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UniProt
- P10809
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途径
- Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
抗原
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