APEX1 抗体 (AA 2-318)
Quick Overview for APEX1 抗体 (AA 2-318) (ABIN3043785)
抗原
See all APEX1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 2-318
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原理
- Anti-APE1/APEX1 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-APE1/APEX1 Antibody Picoband® (ABIN3043785). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human APE1 recombinant protein (Position: P2-L318). Human APE1 shares 94% and 93% amino acid (aa) sequences identity with mouse and rat APE1, respectively.
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亚型
- IgG
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anti-Apurinic/Apyrimidinic Endonuclease 1 (APEX1) (AA 1-318) antibody
APEX1 适用: 人 WB, IF, IHC (p) 宿主: 小鼠 Polyclonal unconjugated
anti-Apurinic/Apyrimidinic Endonuclease 1 (APEX1) (AA 1-242) antibodyAPEX1 适用: 人, 犬, 猴 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 1H9 unconjugated
anti-Apurinic/Apyrimidinic Endonuclease 1 (APEX1) (AA 219-318) antibodyAPEX1 适用: 人, 大鼠, 猴 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 7A2G7 unconjugated
anti-Apurinic/Apyrimidinic Endonuclease 1 (APEX1) (AA 1-318) antibodyAPEX1 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Apurinic/Apyrimidinic Endonuclease 1 (APEX1) (AA 1-318) antibodyAPEX1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Apurinic/Apyrimidinic Endonuclease 1 (APEX1) antibodyAPEX1 适用: 人 WB, IF, IHC (p) 宿主: 小鼠 Monoclonal 1518CT337-123-86-269-232 unconjugated
anti-Apurinic/Apyrimidinic Endonuclease 1 (APEX1) (acLys6) antibodyAPEX1 适用: 人 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Apurinic/Apyrimidinic Endonuclease 1 (APEX1) (N-Term) antibodyAPEX1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Apurinic/Apyrimidinic Endonuclease 1 (APEX1) (AA 1-318) antibodyAPEX1 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Apurinic/Apyrimidinic Endonuclease 1 (APEX1) antibodyAPEX1 适用: 人, 小鼠, 大鼠, 兔 WB, IHC, IF, ICC, ChIP, IP, Neut 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human1. Akiyama, K., Seki, S., Oshida, T., Yoshida, M. C.Structure, promoter analysis and chromosomal assignment of the human APEX gene.Biochim. Biophys. Acta 1219: 15-25, 1994. 2. Chou, K.-M., Cheng, Y.-C.An exonucleolytic activity of human apurinic/apyrimidinic endonuclease on 3-prime mispaired DNA.Nature 415: 655-659, 2002. 3. Robson, C. N., Hochhauser, D., Craig, R., Rack, K., Buckle, V. J., Hickson, I. D.Structure of the human DNA repair gene HAP1 and its localisation to chromosome 14q11.2-12.Nucleic Acids Res. 20: 4417-4421, 1992. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))
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别名
- APEX1
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背景
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Synonyms: DNA- (apurinic or apyrimidinic site) lyase,3.1.-.-,4.2.99.18,APEX nuclease,APEN,Apurinic-apyrimidinic endonuclease 1,AP endonuclease 1,APE-1,REF-1,Redox factor-1,DNA- (apurinic or apyrimidinic site) lyase, mitochondrial,APEX1,APE, APE1, APEX, APX, HAP1, REF1,
Tissue Specificity: Ubiquitously expressed, with highest levels in lung, kidney, heart, gastrointestinal system and prostate. Isoform Testis-specific is expressed in spermatocytes and adult testis. .
Background: APEX1, also called apurinic endonuclease (APE), is a DNA repair enzyme having apurinic/apyrimidinic (AP) endonuclease, 3-prime, 5-prime-exonuclease, DNA 3-prime repair diesterase, and DNA 3-prime-phosphatase activities. The human APEX1 gene consists of 5 exons spanning 2.64 kb and exists as a single copy in the haploid genome. Using in situ hybridization, the APEX1 gene is mapped to 14q11.2-q12. The predicted APEX1 protein, which contained probable nuclear transport signals, was identified as a member of a family of DNA repair enzymes found in lower organisms. The abundance of the large form of APEX1 was increased in leiomyoma extracts relative to myometrial tissue extracts, and the large form was dominant in cell lines derived from leiomyosarcomas. The exonuclease activity of nuclear APEX1 can remove the anti-HIV nucleoside analogs AZT and D4T from the 3-prime terminus of a nick more efficiently than can cytosolic exonucleases.
Sequence Similarities: Belongs to the DNA repair enzymes AP/ExoA family.
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分子量
- 39 kDa
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基因ID
- 328
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UniProt
- P27695
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途径
- DNA Damage Repair, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus
抗原
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