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APEX1 抗体 (AA 2-318)

APEX1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC, FACS 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN3043785
发货至: 中国
  • 抗原 See all APEX1 抗体
    APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))
    抗原表位
    • 7
    • 7
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 2-318
    适用
    • 85
    • 39
    • 37
    • 7
    • 6
    • 6
    • 5
    • 5
    • 5
    • 5
    • 4
    • 3
    • 1
    • 1
    人, 小鼠, 大鼠
    宿主
    • 70
    • 16
    • 1
    克隆类型
    • 69
    • 18
    多克隆
    标记
    • 58
    • 4
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This APEX1 antibody is un-conjugated
    应用范围
    • 74
    • 27
    • 26
    • 26
    • 18
    • 12
    • 7
    • 6
    • 4
    • 3
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)
    原理
    Anti-APE1/APEX1 Antibody Picoband®
    交叉反应 (详细)
    No cross-reactivity with other proteins
    产品特性
    Anti-APE1/APEX1 Antibody Picoband® (ABIN3043785). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
    纯化方法
    Immunogen affinity purified.
    免疫原
    E.coli-derived human APE1 recombinant protein (Position: P2-L318). Human APE1 shares 94% and 93% amino acid (aa) sequences identity with mouse and rat APE1, respectively.
    亚型
    IgG
    Top Product
    Discover our top product APEX1 Primary Antibody
  • 应用备注
    Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
    Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
    Immunofluorescence, 2 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human1. Akiyama, K., Seki, S., Oshida, T., Yoshida, M. C.Structure, promoter analysis and chromosomal assignment of the human APEX gene.Biochim. Biophys. Acta 1219: 15-25, 1994. 2. Chou, K.-M., Cheng, Y.-C.An exonucleolytic activity of human apurinic/apyrimidinic endonuclease on 3-prime mispaired DNA.Nature 415: 655-659, 2002. 3. Robson, C. N., Hochhauser, D., Craig, R., Rack, K., Buckle, V. J., Hickson, I. D.Structure of the human DNA repair gene HAP1 and its localisation to chromosome 14q11.2-12.Nucleic Acids Res. 20: 4417-4421, 1992.
    说明

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    浓度
    500 μg/mL
    缓冲液
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    4 °C,-20 °C
    储存方法
    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • 抗原
    APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))
    别名
    APEX1 (APEX1 产品)
    背景

    Synonyms: DNA- (apurinic or apyrimidinic site) lyase,3.1.-.-,4.2.99.18,APEX nuclease,APEN,Apurinic-apyrimidinic endonuclease 1,AP endonuclease 1,APE-1,REF-1,Redox factor-1,DNA- (apurinic or apyrimidinic site) lyase, mitochondrial,APEX1,APE, APE1, APEX, APX, HAP1, REF1,

    Tissue Specificity: Ubiquitously expressed, with highest levels in lung, kidney, heart, gastrointestinal system and prostate. Isoform Testis-specific is expressed in spermatocytes and adult testis. .

    Background: APEX1, also called apurinic endonuclease (APE), is a DNA repair enzyme having apurinic/apyrimidinic (AP) endonuclease, 3-prime, 5-prime-exonuclease, DNA 3-prime repair diesterase, and DNA 3-prime-phosphatase activities. The human APEX1 gene consists of 5 exons spanning 2.64 kb and exists as a single copy in the haploid genome. Using in situ hybridization, the APEX1 gene is mapped to 14q11.2-q12. The predicted APEX1 protein, which contained probable nuclear transport signals, was identified as a member of a family of DNA repair enzymes found in lower organisms. The abundance of the large form of APEX1 was increased in leiomyoma extracts relative to myometrial tissue extracts, and the large form was dominant in cell lines derived from leiomyosarcomas. The exonuclease activity of nuclear APEX1 can remove the anti-HIV nucleoside analogs AZT and D4T from the 3-prime terminus of a nick more efficiently than can cytosolic exonucleases.

    Sequence Similarities: Belongs to the DNA repair enzymes AP/ExoA family.

    分子量
    39 kDa
    基因ID
    328
    UniProt
    P27695
    途径
    DNA Damage Repair, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus
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