ATG14 抗体 (N-Term)
Quick Overview for ATG14 抗体 (N-Term) (ABIN3043721)
抗原
See all ATG14 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 70-101, N-Term
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原理
- Anti-ATG14L Antibody Picoband®
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序列
- RDRERFIDKK ERLSRLKSKQ EEFQKEVLKA ME
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-ATG14L Antibody Picoband® (ABIN3043721). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the N-terminus of human ATG14L, different from the related mouse and rat sequences by two amino acids.
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亚型
- IgG
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anti-ATG14 Autophagy Related 14 (ATG14) (AA 43-303) antibody
ATG14 适用: 人 ELISA, IHC 宿主: 小鼠 Monoclonal 1F7C10 unconjugated
anti-ATG14 Autophagy Related 14 (ATG14) antibodyATG14 适用: 人, 大鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-ATG14 Autophagy Related 14 (ATG14) (AA 1-492) antibodyATG14 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-ATG14 Autophagy Related 14 (ATG14) (AA 1-310) antibodyATG14 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-ATG14 Autophagy Related 14 (ATG14) (AA 1-310) antibodyATG14 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-ATG14 Autophagy Related 14 (ATG14) (AA 162-190) antibodyATG14 适用: 人 WB, FACS 宿主: 兔 Polyclonal RB19671 unconjugated
anti-ATG14 Autophagy Related 14 (ATG14) (AA 55-84), (N-Term) antibodyATG14 适用: 人, 小鼠 WB, FACS 宿主: 兔 Polyclonal RB19669 unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human1. Diao J, Liu R, Rong Y, Zhao M, Zhang J, Lai Y, Zhou Q, Wilz LM, Li J, Vivona S, Pfuetzner RA, Brunger AT, Zhong Q. ""ATG14 promotes membrane tethering and fusion of autophagosomes to endolysosomes"". Nature, 2015 Apr 23. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- ATG14 (ATG14 Autophagy Related 14 (ATG14))
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别名
- ATG14
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背景
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Synonyms: Beclin 1-associated autophagy-related key regulator ,Barkor ,Autophagy-related protein 14-like protein ,Atg14L ,ATG14 ,ATG14L , KIAA0831,
Tissue Specificity: Widely expressed, especially in kidney, liver, lymph nodes and bone marrow. .
Background: ATG14 (also known as beclin-1-associated autophagy-related key regulator (Barkor) or ATG14L), an essential autophagy-specific regulator of the class III phosphatidylinositol 3-kinase complex, promotes membrane tethering of protein-free liposomes, and enhances hemifusion and full fusion of proteoliposomes reconstituted with the target (t)-SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) syntaxin 17 (STX17) and SNAP29, and the vesicle (v)-SNARE VAMP8 (vesicle-associated membrane protein 8). ATG14 binds to the SNARE core domain of STX17 through its coiled-coil domain, and stabilizes the STX17-SNAP29 binary t-SNARE complex on autophagosomes.
Sequence Similarities: Belongs to the ATG14 family.
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分子量
- 59 kDa
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基因ID
- 22863
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途径
- Autophagy
抗原
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