ATG14 抗体 (N-Term)
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北京 101111
Quick Overview for ATG14 抗体 (N-Term) (ABIN3043721)
抗原
See all ATG14 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 70-101, N-Term
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原理
- Anti-ATG14L Antibody
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序列
- RDRERFIDKK ERLSRLKSKQ EEFQKEVLKA ME
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-ATG14L Antibody (ABIN3043721). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the N-terminus of human ATG14L, different from the related mouse and rat sequences by two amino acids.
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亚型
- IgG
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anti-ATG14 Autophagy Related 14 (ATG14) (AA 43-303) antibody
ATG14 适用: 人 ELISA, IHC 宿主: 小鼠 Monoclonal 1F7C10 unconjugated
anti-ATG14 Autophagy Related 14 (ATG14) (AA 1-492) antibodyATG14 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-ATG14 Autophagy Related 14 (ATG14) (AA 1-310) antibodyATG14 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-ATG14 Autophagy Related 14 (ATG14) (AA 55-84), (N-Term) antibodyATG14 适用: 人, 小鼠 WB, FACS 宿主: 兔 Polyclonal RB19669 unconjugated
anti-ATG14 Autophagy Related 14 (ATG14) (AA 162-190) antibodyATG14 适用: 人 WB, FACS 宿主: 兔 Polyclonal RB19671 unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- ATG14 (ATG14 Autophagy Related 14 (ATG14))
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别名
- ATG14
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背景
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Synonyms: Beclin 1-associated autophagy-related key regulator ,Barkor ,Autophagy-related protein 14-like protein ,Atg14L ,ATG14 ,ATG14L , KIAA0831,
Tissue Specificity: Widely expressed, especially in kidney, liver, lymph nodes and bone marrow. .
Background: ATG14 (also known as beclin-1-associated autophagy-related key regulator (Barkor) or ATG14L), an essential autophagy-specific regulator of the class III phosphatidylinositol 3-kinase complex, promotes membrane tethering of protein-free liposomes, and enhances hemifusion and full fusion of proteoliposomes reconstituted with the target (t)-SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) syntaxin 17 (STX17) and SNAP29, and the vesicle (v)-SNARE VAMP8 (vesicle-associated membrane protein 8). ATG14 binds to the SNARE core domain of STX17 through its coiled-coil domain, and stabilizes the STX17-SNAP29 binary t-SNARE complex on autophagosomes.
Sequence Similarities: Belongs to the ATG14 family.
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分子量
- 59 kDa
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基因ID
- 22863
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途径
- Autophagy
抗原
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