PKLR 抗体 (C-Term)
Quick Overview for PKLR 抗体 (C-Term) (ABIN3043607)
抗原
See all PKLR 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- AA 522-552, C-Term
-
原理
- Anti-PKLR Antibody Picoband®
-
序列
- EAIWADDVDR RVQFGIESGK LRGFLRVGDL V
-
交叉反应 （详细）
- No cross-reactivity with other proteins
-
产品特性
- Anti-PKLR Antibody Picoband® (ABIN3043607). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
-
纯化方法
- Immunogen affinity purified.
-
免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human PKLR, different from the related mouse sequence by one amino acid, and identical to the rat sequence.
-
亚型
- IgG
-
-
anti-Pyruvate Kinase, Liver and RBC (PKLR) (AA 219-420) antibody
PKLR 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Kinase, Liver and RBC (PKLR) (AA 114-323) antibodyPKLR 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Kinase, Liver and RBC (PKLR) (AA 1-574) antibodyPKLR 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Kinase, Liver and RBC (PKLR) (AA 1-574) antibodyPKLR 适用: 人 WB, IHC (p) 宿主: 小鼠 Polyclonal unconjugated
anti-Pyruvate Kinase, Liver and RBC (PKLR) (Internal Region) antibodyPKLR 适用: 人, 小鼠, 大鼠 WB, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Kinase, Liver and RBC (PKLR) (N-Term) antibodyPKLR 适用: 人, 小鼠, 大鼠, 犬, C. elegans WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Kinase, Liver and RBC (PKLR) (N-Term) antibodyPKLR 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 兔, 马, Pig, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Kinase, Liver and RBC (PKLR) (AA 56-105) antibodyPKLR 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 兔, Bat, 猴, 非洲爪蟾 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Pyruvate Kinase, Liver and RBC (PKLR) (AA 521-551), (C-Term) antibodyPKLR 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB3694 unconjugated
anti-Pyruvate Kinase, Liver and RBC (PKLR) (AA 1-30), (N-Term) antibodyPKLR 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB03692 unconjugated
-
-
应用备注
-
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. "Entrez Gene: PKLR pyruvate kinase, liver and RBC". 2. Tani K, Fujii H, Tsutsumi H, Sukegawa J, Toyoshima K, Yoshida MC, Noguchi T, Tanaka T, Miwa S (Apr 1987). "Human liver type pyruvate kinase: cDNA cloning and chromosomal assignment". Biochem Biophys Res Commun 143 (2): 431-8. -
说明
-
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
-
浓度
- 500 μg/mL
-
缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Avoid repeated freezing and thawing.
-
储存条件
- 4 °C,-20 °C
-
储存方法
-
Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
-
-
- PKLR (Pyruvate Kinase, Liver and RBC (PKLR))
-
别名
- PKLR
-
背景
-
Synonyms: Pyruvate kinase PKLR,2.7.1.40,Pyruvate kinase 1,Pyruvate kinase isozymes L/R,R-type/L-type pyruvate kinase,Red cell/liver pyruvate kinase,PKLR,PK1, PKL,
Tissue Specificity: Ubiquitous, expressed at low levels. Not detectable in a number of hepatocarcinoma cell lines.
Background: Pyruvate kinase isozymes R/L is an enzyme that in humans is encoded by the PKLR gene. It is mapped to 1q21. The protein encoded by this gene is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene.
Sequence Similarities: Belongs to the pyruvate kinase family.
-
分子量
- 62 kDa
-
基因ID
- 5313
-
UniProt
- P30613
-
途径
- Ribonucleoside Biosynthetic Process
抗原
-
