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- 抗原 See all POLB 抗体
- POLB (Polymerase (DNA Directed), beta (POLB))
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抗原表位
- AA 122-335
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This POLB antibody is un-conjugated
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应用范围
- Western Blotting (WB)
- 原理
- Rabbit IgG polyclonal antibody for DNA polymerase beta(POLB) detection. Tested with WB in Human.
- 交叉反应 （详细）
- No cross reactivity with other proteins.
- 产品特性
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Rabbit IgG polyclonal antibody for DNA polymerase beta(POLB) detection. Tested with WB in Human.
Gene Name: polymerase (DNA directed), beta
Protein Name: DNA polymerase beta - 纯化方法
- Immunogen affinity purified.
- 免疫原
- E.coli-derived human POLB recombinant protein (Position: L122-E335). Human POLB shares 95.3% and 94.9% amino acid (aa) sequence identity with mouse and rat POLB, respectively.
- 亚型
- IgG
- Top Product
- Discover our top product POLB Primary Antibody
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anti-Polymerase (DNA Directed), beta (POLB) (AA 1-335) antibody
POLB 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), beta (POLB) (AA 1-335) antibodyPOLB 适用: 人 WB, IF, IHC (p) 宿主: 小鼠 Polyclonal unconjugated
anti-Polymerase (DNA Directed), beta (POLB) (C-Term) antibodyPOLB 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), beta (POLB) (AA 1-300) antibodyPOLB 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), beta (POLB) (C-Term) antibodyPOLB 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), beta (POLB) (AA 55-160) antibodyPOLB 适用: 小鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), beta (POLB) (C-Term) antibodyPOLB 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), beta (POLB) (C-Term) antibodyPOLB 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Polymerase (DNA Directed), beta (POLB) (AA 224-333) antibodyPOLB 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Polymerase (DNA Directed), beta (POLB) (AA 1-335) antibodyPOLB 适用: 人 IP 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users. - 说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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- 抗原
- POLB (Polymerase (DNA Directed), beta (POLB))
- 别名
- POLB (POLB 产品)
- 别名
- wu:fa97a05 antibody, zgc:109924 antibody, POLB antibody, DDBDRAFT_0186152 antibody, DDBDRAFT_0215784 antibody, DDBDRAFT_0232376 antibody, DDB_0186152 antibody, DDB_0215784 antibody, DDB_0232376 antibody, A430088C08Rik antibody, polymerase (DNA directed), beta antibody, DNA polymerase beta antibody, DNA polymerase X family protein antibody, polymerase (DNA directed), beta S homeolog antibody, polb antibody, POLB antibody, polB antibody, polb.S antibody, Polb antibody
- 背景
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Polymerase (DNA directed), beta, also known as POLB, is an enzyme that, in humans, is encoded by the POLB gene. It is localized on 8p11.2. The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. It is found that a truncated POLB is expressed in primary colorectal tumors and inhibits the normal repair function of wildtype POLB. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date. Additionally, human POLB forms a complex with and is methylated by PRMT6. In vitro, methylated POLB possesses significantly higher DNA polymerase activity when compared to that of unmodified enzyme. The increase in DNA polymerase activity upon methylation is due to the enhanced DNA binding and processivity of POLB.
Synonyms: DNA directed DNA polymerase beta antibody|DNA pol beta antibody|DNA polymerase beta antibody|DNA polymerase beta subunit antibody| DPOLB_HUMAN antibody|MGC125976 antibody|Pol B antibody|Pol beta antibody|POLB antibody|Polymerase (DNA directed) beta antibody - 基因ID
- 5423
- UniProt
- P06746
- 途径
- DNA Damage Repair
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