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POLB 抗体 (AA 122-335)

This anti-POLB antibody is a 兔多克隆 antibody detecting POLB in WB. Suitable for 人.

Anti- POLB Picoband antibody, Western blottingAll lanes: Anti POLB at 0. (POLB 抗体 (AA 122-335))

1 image

产品编号 ABIN3043545

发货至: 中国

Quick Overview for POLB 抗体 (AA 122-335) (ABIN3043545)

抗原

See all POLB 抗体

POLB (Polymerase (DNA Directed), beta (POLB))

适用

45
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27
9
3
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1

人

宿主

兔

克隆类型

多克隆

标记

28
4
3
2
2
2
2
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2
1
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1
1
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1

This POLB antibody is un-conjugated

应用范围

38
21
19
14
13
6
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4
3

Western Blotting (WB)

Discover our top product POLB Primary Antibody

抗原表位
15

6

5

3

3

1

1

1

1

1

1

1

1

1
AA 122-335

原理

Anti-DNA Polymerase beta/POLB Antibody Picoband®

交叉反应（详细）

No cross-reactivity with other proteins.

产品特性

Anti-DNA Polymerase beta/POLB Antibody Picoband® (ABIN3043545). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

纯化方法

Immunogen affinity purified.

免疫原

E.coli-derived human POLB recombinant protein (Position: L122-E335). Human POLB shares 95.3% and 94.9% amino acid (aa) sequence identity with mouse and rat POLB, respectively.

亚型

IgG
anti-Polymerase (DNA Directed), beta (POLB) (AA 1-335) antibody
POLB 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Polymerase (DNA Directed), beta (POLB) (C-Term) antibody
POLB 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Polymerase (DNA Directed), beta (POLB) (AA 1-335) antibody
POLB 适用: 人 WB, IF, IHC (p) 宿主: 小鼠 Polyclonal unconjugated

anti-Polymerase (DNA Directed), beta (POLB) (AA 1-300) antibody
POLB 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Polymerase (DNA Directed), beta (POLB) (AA 55-160) antibody
POLB 适用: 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Polymerase (DNA Directed), beta (POLB) (C-Term) antibody
POLB 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Polymerase (DNA Directed), beta (POLB) (C-Term) antibody
POLB 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Polymerase (DNA Directed), beta (POLB) (C-Term) antibody
POLB 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Polymerase (DNA Directed), beta (POLB) (AA 224-333) antibody
POLB 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated

anti-Polymerase (DNA Directed), beta (POLB) (AA 1-335) antibody
POLB 适用: 人 IP 宿主: 兔 Polyclonal unconjugated

应用备注

Western blot, 0.1-0.5 μg/mL, Human
1. "Entrez Gene: POLB polymerase (DNA ed), beta". 2. Bhattacharyya, N., Banerjee, S. A variant of DNA polymerase beta acts as a dominant negative mutant. Proc. Nat. Acad. Sci. 94: 10324-10329, 1997. 3. El-Andaloussi, N., Valovka, T., Toueille, M., Steinacher, R., Focke, F., Gehrig, P., Covic, M., Hassa, P. O., Schar, P., Hubscher, U., Hottiger, M. O. Arginine methylation regulates DNA polymerase beta. Molec. Cell 22: 51-62, 2006.

说明

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

限制

仅限研究用
状态

Lyophilized

溶解方式

Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

浓度

500 μg/mL

缓冲液

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C,-20 °C

储存方法

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
抗原

POLB (Polymerase (DNA Directed), beta (POLB))

别名

POLB

背景

Synonyms: DNA polymerase beta,2.7.7.7,4.2.99.-,POLB,
Tissue Specificity: Major component of lens fiber gap junctions.
Background: Polymerase (DNA ed), beta, also known as POLB, is an enzyme that, in humans, is encoded by the POLB gene. It is localized on 8p11.2. The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. It is found that a truncated POLB is expressed in primary colorectal tumors and inhibits the normal repair function of wildtype POLB. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date. Additionally, human POLB forms a complex with and is methylated by PRMT6. In vitro, methylated POLB possesses significantly higher DNA polymerase activity when compared to that of unmodified enzyme. The increase in DNA polymerase activity upon methylation is due to the enhanced DNA binding and processivity of POLB.

分子量

39 kDa

基因ID

5423

UniProt

P06746

途径

DNA Damage Repair

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