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- 抗原 See all MCAM 抗体
- MCAM (Melanoma Cell Adhesion Molecule (MCAM))
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抗原表位
- AA 59-401
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This MCAM antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 原理
- Rabbit IgG polyclonal antibody for Cell surface glycoprotein MUC18(MCAM) detection. Tested with WB, IHC-P in Human.
- 交叉反应 （详细）
- No cross reactivity with other proteins.
- 产品特性
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Rabbit IgG polyclonal antibody for Cell surface glycoprotein MUC18(MCAM) detection. Tested with WB, IHC-P in Human.
Gene Name: melanoma cell adhesion molecule
Protein Name: Cell surface glycoprotein MUC18 - 纯化方法
- Immunogen affinity purified.
- 免疫原
- E.coli-derived human CD146 recombinant protein (Position: H59-A401). Human CD146 shares 73% amino acid (aa) sequence identity with both mouse and rat CD146.
- 亚型
- IgG
- Top Product
- Discover our top product MCAM Primary Antibody
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anti-Melanoma Cell Adhesion Molecule (MCAM) (AA 84-189) antibody
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anti-Melanoma Cell Adhesion Molecule (MCAM) antibodyMCAM 适用: 人 FACS, IHC, WB, IF, ELISA 宿主: 兔 Monoclonal 8A10 unconjugated Recombinant Antibody
anti-Melanoma Cell Adhesion Molecule (MCAM) antibodyMCAM 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Melanoma Cell Adhesion Molecule (MCAM) (AA 226-374) antibodyMCAM 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal MCAM-3046 unconjugated
anti-Melanoma Cell Adhesion Molecule (MCAM) (AA 1-646) antibodyMCAM 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Melanoma Cell Adhesion Molecule (MCAM) (AA 201-300) antibodyMCAM 适用: 人, 大鼠 FACS, WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Melanoma Cell Adhesion Molecule (MCAM) antibodyMCAM 适用: 人, 小鼠, 大鼠 IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Melanoma Cell Adhesion Molecule (MCAM) antibodyMCAM 适用: 人 IHC, WB, ELISA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for CD146 is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. - 说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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- 抗原
- MCAM (Melanoma Cell Adhesion Molecule (MCAM))
- 别名
- MCAM (MCAM 产品)
- 别名
- CD146 antibody, MUC18 antibody, Muc18 antibody, 1-gicerin antibody, AV025631 antibody, CD149 antibody, s-endo antibody, s-gicerin antibody, cd146 antibody, gicerin antibody, id:ibd3512 antibody, mcam antibody, MCAM antibody, melanoma cell adhesion molecule antibody, melanoma cell adhesion molecule b antibody, MCAM antibody, Mcam antibody, mcamb antibody
- 背景
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CD146 (cluster of differentiation 146), also known as the melanoma cell adhesion molecule (MCAM) or cell surface glycoprotein MUC18, is a 113 kDa cell adhesion molecule currently used as a marker for endothelial cell lineage. MCAM, a member of the immunoglobulin superfamily, is homologous to several cell adhesion molecules and is associated with tumor progression and the development of metastasis in human malignant melanoma. By radiation hybrid analysis, this gene is mapped to chromosome 11q23.3. MCAM has been demonstrated to appear on a small subset of T and B lymphocytes in the peripheral blood of healthy individuals. MCAM has been seen as a marker for mesenchymal stem cells isolated from multiple adult and fetal organs, and its expression may be linked to multipotency mesenchymal stem cells with greater differentiation potential express higher levels of MCAM on the cell surface.
Synonyms: A32 antigen antibody|CD 146 antibody|CD146 antibody|CD146 antigen antibody|Cell surface glycoprotein MUC18 antibody|Cell surface glycoprotein P1H12 antibody|Gicerin antibody|MCAM antibody|Melanoma adhesion molecule antibody|Melanoma associated antigen A32 antibody|Melanoma associated antigen MUC18 antibody|Melanoma associated glycoprotein MUC18 antibody|Melanoma cell adhesion molecule antibody|Melanoma-associated antigen A32 antibody|Melanoma-associated antigen MUC18 antibody|MelCAM antibody|MUC 18 antibody|MUC18 antibody|MUC18_HUMAN antibody|S endo 1 antibody|S endo 1 endothelial associated antigen antibody|S-endo 1 endothelial-associated antigen antibody|Sendo 1 endothelial associated antigen antibody|Sendo1 antibody - 基因ID
- 4162
- UniProt
- P43121
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