RAC1 抗体 (C-Term)
(2 references)Quick Overview for RAC1 抗体 (C-Term) (ABIN3043366)
抗原
See all RAC1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 169-189, C-Term
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原理
- Anti-Rac1 Antibody Picoband®
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序列
- FDEAIRAVLC PPPVKKRKRK C
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-Rac1 Antibody Picoband® (ABIN3043366). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human Rac1, identical to the related mouse and rat sequences.
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亚型
- IgG
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anti-RAC1 (Center) antibody
适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-RAC1 (AA 1-192) antibody适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-RAC1 antibody适用: 人, 大鼠 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 1301CT276-121-104 unconjugated
anti-RAC1 (AA 11-185) antibody适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-RAC1 antibody适用: 人, 大鼠 WB, IHC (p), FACS 宿主: 小鼠 Monoclonal 1301CT276-121-104 unconjugated
anti-RAC1 (pSer71) antibody适用: 人, 小鼠, 大鼠 WB, IF (cc), IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-RAC1 (AA 101-192) antibody适用: 人, 小鼠 ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-RAC1 antibody适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 小鼠 Monoclonal 13C4 unconjugated
anti-RAC1 (AA 49-78) antibody适用: 人, 大鼠 WB 宿主: 兔 Polyclonal RB11123 unconjugated
anti-RAC1 (Middle Region) antibody适用: 人, 小鼠, 大鼠, 犬, 斑马鱼, 绵羊, 兔, 马, Cow, 豚鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human
1. Didsbury J, Weber RF, Bokoch GM, Evans T, Snyderman R (October 1989). "rac, a novel ras-related family of proteins that are botulinum toxin substrates". J. Biol. Chem. 264 (28): 16378-82. 2. Jordan P, Brazåo R, Boavida MG, Gespach C, Chastre E (November 1999). "Cloning of a novel human Rac1b splice variant with increased expression in colorectal tumors".Oncogene 18 (48): 6835-9. 3. Zhou C, Licciulli S, Avila JL, Cho M, Troutman S, Jiang P, Kossenkov AV, Showe LC, Liu Q, Vachani A, Albelda SM, Kissil JL (February 2013). "The Rac1 splice form Rac1b promotes K-ras-induced lung tumorigenesis". Oncogene 32 (7): 903-9. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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: "Novel derivative of Paeonol, Paeononlsilatie sodium, alleviates behavioral damage and hippocampal dendritic injury in Alzheimer's disease concurrent with cofilin1/phosphorylated-cofilin1 and ..." in: PLoS ONE, Vol. 12, Issue 9, pp. e0185102, (2017) (PubMed).
: "Gadolinium promoted proliferation in mouse embryo fibroblast NIH3T3 cells through Rac and PI3K/Akt signaling pathways." in: Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine, Vol. 27, Issue 4, pp. 753-62, (2015) (PubMed).
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: "Novel derivative of Paeonol, Paeononlsilatie sodium, alleviates behavioral damage and hippocampal dendritic injury in Alzheimer's disease concurrent with cofilin1/phosphorylated-cofilin1 and ..." in: PLoS ONE, Vol. 12, Issue 9, pp. e0185102, (2017) (PubMed).
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- RAC1
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背景
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Synonyms: Ras-related C3 botulinum toxin substrate 1,Cell migration-inducing gene 5 protein,Ras-like protein TC25,p21-Rac1,RAC1,TC25,MIG5,
Tissue Specificity: Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. Its expression is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues.
Background: Rac1, also known as Ras-related C3 botulinum toxin substrate 1, is a protein found in human cells. It is encoded by the RAC1gene. This gene can produce a variety of alternatively splicedversions of the Rac1 protein, which appear to carry out different functions. This gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene.
Sequence Similarities: Belongs to the small GTPase superfamily. Rho family.
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分子量
- 21 kDa
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基因ID
- 5879
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UniProt
- P63000
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途径
- WNT signaling, Regulation of Actin Filament Polymerization, Cell-Cell Junction Organization, Thromboxane A2 Receptor Signaling, VEGF Signaling
抗原
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