Nibrin 抗体 (C-Term)
Quick Overview for Nibrin 抗体 (C-Term) (ABIN3043282)
抗原
See all Nibrin (NBN) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 714-745, C-Term
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原理
- Anti-p95 NBS1/NBN Antibody Picoband®
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序列
- RKNTELEEWL RQEMEVQNQH AKEESLADDL FR
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-p95 NBS1/NBN Antibody Picoband® (ABIN3043282). Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human p95 NBS1, different from the related mouse sequence by three amino acids, and from the related rat sequence by five amino acids.
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亚型
- IgG
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anti-Nibrin (NBN) (AA 467-615) antibody
NBN 适用: 人 WB, ELISA, IHC, ICC, FACS 宿主: 小鼠 Monoclonal 7E4C2 unconjugated
anti-Nibrin (NBN) (AA 183-460) antibodyNBN 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 8E7 unconjugated
anti-Nibrin (NBN) (AA 461-754) antibodyNBN 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 3A9 unconjugated
anti-Nibrin (NBN) (AA 461-754) antibodyNBN 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 3A2 unconjugated
anti-Nibrin (NBN) (AA 183-460) antibodyNBN 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 6E11 unconjugated
anti-Nibrin (NBN) (AA 183-460) antibodyNBN 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 2F5 unconjugated
anti-Nibrin (NBN) (AA 435-754) antibodyNBN 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nibrin (NBN) (AA 645-754) antibodyNBN 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3E4 unconjugated
anti-Nibrin (NBN) antibodyKO Validated NBN 适用: 人 WB, IF, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Nibrin (NBN) (pSer343) antibodyNBN 适用: 小鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry, 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 5 μg/mL
1. "Atlas of Genetics and Cytogenetics in Oncology and Haematology - NBS1". 2. Carney JP, Maser RS, Olivares H, Davis EM, Le Beau M, Yates JR, Hays L, Morgan WF, Petrini JH (May 1998). "The hMre11/hRad50 protein complex and Nijmegen breakage syndrome: linkage of double-strand break repair to the cellular DNA damage response". Cell 93 (3): 477-86. 3. Varon R, Vissinga C, Platzer M, Cerosaletti KM, Chrzanowska KH, Saar K, Beckmann G, Seemanová E, Cooper PR, Nowak NJ, Stumm M, Weemaes CM, Gatti RA, Wilson RK, Digweed M, Rosenthal A, Sperling K, Concannon P, Reis A (May 1998). "Nibrin, a novel DNA double-strand break repair protein, is mutated in Nijmegen breakage syndrome". Cell 93 (3): 467-76. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- Nibrin (NBN)
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别名
- NBN
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背景
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Synonyms: Nibrin,Cell cycle regulatory protein p95,Nijmegen breakage syndrome protein 1,NBN,NBS, NBS1, P95,
Tissue Specificity: Ubiquitous. Expressed at high levels in testis.
Background: p95 NBS1, also known as NBN or Nibrin, is a protein which in humans is encoded by the NBN gene. Nibrin is a protein associated with the repair of double strand breaks (DSBs) which pose serious damage to a genome. It is a 754 amino acid protein identified as a member of the NBS1/hMre11/RAD50 (N/M/R, more commonly referred to asMRN) double strand DNA break repair complex. This complex recognizes DNA damage and rapidly relocates to DSB sites and forms nuclear foci. It also has a role in regulation of N/M/R (MRN) protein complex activity which includes end-processing of both physiological and mutagenic DNA double strand breaks (DSBs).
Sequence Similarities: Contains 1 BRCT domain.
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分子量
- 95 kDa
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基因ID
- 4683
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UniProt
- O60934
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途径
- DNA Damage Repair, Production of Molecular Mediator of Immune Response
抗原
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