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PKC alpha 抗体 (N-Term)

Name: PKC alpha 抗体 (N-Term)
SKU: ABIN3043025
Availability: OutOfStock

PKCa 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

Anti-PKC alpha antibody, Western blotting WB: HELA Cell Lysate (PKC alpha 抗体 (N-Term))

Anti-PKC alpha antibody, IHC(P) IHC(P): Human Mammary Cancer Tissue (PKC alpha 抗体 (N-Term))

IHC

3 images

(5 references)

产品编号 ABIN3043025

发货至: 中国

Datasheet as PDF

抗原 See all PKC alpha (PKCa) 抗体

PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))
抗原表位
24

20

18

12

11

10

8

7

7

6

6

5

5

5

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 153-182, N-Term
适用
178

90

68

6

5

5

3

2

2

1

1

1

1
人
宿主
174

12

1
兔
Compatible Secondaries
克隆类型
140

46
多克隆
标记
96

15

13

6

6

6

6

6

6

6

6

6

2

2

1

1

1

1

1
This PKC alpha antibody is un-conjugated
应用范围
128

71

53

33

22

20

17

15

14

8

5

2

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

原理

Rabbit IgG polyclonal antibody for Protein kinase C alpha type(PRKCA) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

序列

MDHTEKRGRI YLKAEVADEK LHVTVRDAKN

交叉反应（详细）

Predicted Cross Reactivity: mouse, rat
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

产品特性

Rabbit IgG polyclonal antibody for Protein kinase C alpha type(PRKCA) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: protein kinase C, alpha
Protein Name: Protein kinase C alpha type

纯化方法

Immunogen affinity purified.

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human PRKCA (153-182aa MDHTEKRGRIYLKAEVADEKLHVTVRDAKN), different from the related rat and mouse sequences by one amino acid.

亚型

IgG

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anti-Protein Kinase C, alpha (PKCa) antibody
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anti-Protein Kinase C, alpha (PKCa) antibody
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PKCa 适用: 人 WB, FACS, IHC (p), IF 宿主: 兔 Polyclonal RB01012 unconjugated

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PKCa 适用: 人 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Protein Kinase C, alpha (PKCa) (C-Term) antibody
PKCa 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Protein Kinase C, alpha (PKCa) (N-Term) antibody
PKCa 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

应用备注

WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

说明

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

限制

仅限研究用
状态

Lyophilized

溶解方式

Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

浓度

500 μg/mL

缓冲液

Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

储存液

Thimerosal (Merthiolate), Sodium azide

注意事项

This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C/-20 °C

储存方法

At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Yan-Fang, Zhi-Heng, Li-Xiao, Fang, Jun, Gang, Lan, Na-Na, Xiao-Juan, Li-Chao, Wen-Li, Pei-Fang, He, Guang-Hao, Yan-Hong, Yi-Ping, Yun-Yun, Hui-Ting, Yi, Mei-Fang, Lin, Jian, Shao-Yan, Xue-Ming, Xing et al.: "Molecular Mechanism of the Cell Death Induced by the Histone Deacetylase Pan Inhibitor LBH589 (Panobinostat) in Wilms Tumor Cells. ..." in: PLoS ONE, Vol. 10, Issue 7, pp. e0126566, (2016) (PubMed).

Gan, Wang, Zhao, Wu, Yang, Peng: "5-(Bis(3-(2-hydroxyethyl)-1H-indol-2-yl)methyl)-2-hydroxybenzoic acid (BHIMHA): showing a strategy of designing drug to block lung metastasis of tumors." in: Drug design, development and therapy, Vol. 10, pp. 711-21, (2016) (PubMed).

Cheng, Gu, Yuan, Feng, Jia: "Suppression of A549 cell proliferation and metastasis by calycosin via inhibition of the PKC-α/ERK1/2 pathway: An in vitro investigation." in: Molecular medicine reports, Vol. 13, Issue 4, pp. 3709-10, (2016) (PubMed).

Sun, Li, Ma, Liu, Xu, Ye, Hou, Wang, Li, Jiang: "The predictive value and potential mechanisms of miRNA-328 and miRNA-378 for brain metastases in operable and advanced non-small-cell lung cancer." in: Japanese journal of clinical oncology, Vol. 45, Issue 5, pp. 464-73, (2015) (PubMed).

Cheng, Gu, Zhang, Yuan, Zhao, Jiang, Jia: "Astragaloside IV inhibits migration and invasion in human lung cancer A549 cells via regulating PKC-?-ERK1/2-NF-?B pathway." in: International immunopharmacology, Vol. 23, Issue 1, pp. 304-13, (2015) (PubMed).
抗原

PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

别名

PRKCA (PKCa 产品)

别名

pkc-alpha antibody, pkcalpha antibody, im:7139045 antibody, si:dkey-8l19.1 antibody, LOC100219195 antibody, AAG6 antibody, PKC-alpha antibody, PKCA antibody, PRKACA antibody, AI875142 antibody, Pkca antibody, PKRCA antibody, protein kinase C alpha antibody, protein kinase C, alpha L homeolog antibody, protein kinase C, alpha antibody, PRKCA antibody, prkca.L antibody, prkca antibody, Prkca antibody

背景

Protein kinase C(PKC) is the major phorbol ester receptor. Activation of PKC by calcium ions and the second messenger diacylglycerol is though to play a central role in the induction of cellular responses to a variety of ligand-receptor systems and in the regulation of cellular responsiveness to external stimuli. Three of these, termed alpha, beta and gamma, are highly homologous. PRKCA1 is mapped to 17q22-q23.2. PKC-alpha regulates cardiac contractility and propensity toward heart failure.

Synonyms: AAG6 antibody|Aging associated gene 6 antibody|KPCA_HUMAN antibody|MGC129900 antibody|MGC129901 antibody|PICK 1 antibody|PKC A antibody|PKC alpha antibody|PKC alpha antibody|PKC-A antibody|PKC-alpha antibody|PKCA antibody|PRKACA antibody|PRKC A antibody|PRKCA antibody|Protein Kinase C alpha antibody|Protein kinase C alpha type antibody

UniProt

P17252

途径

WNT signaling, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling